Site-Specific PEGylation of Engineered Cysteine Analogues of Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor

• Published in: Bioconjugate chemistry Vol. 16; no. 5; pp. 1291 - 1298
• Main Authors: Doherty, Daniel H, Rosendahl, Mary S, Smith, Darin J, Hughes, Jennifer M, Chlipala, Elizabeth A, Cox, George N
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.09.2005
• Subjects: Animals; Cell Line; Cysteine - analogs & derivatives; Cysteine - chemistry; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression; Granulocyte-Macrophage Colony-Stimulating Factor - blood; Granulocyte-Macrophage Colony-Stimulating Factor - chemistry; Granulocyte-Macrophage Colony-Stimulating Factor - genetics; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacokinetics; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Humans; Leukocytes; Male; Pharmacology; Polyethylene Glycols - chemistry; Protein Sorting Signals; Proteins; Rats; Rats, Sprague-Dawley; Recombinant Proteins

Granulocyte macrophage colony-stimulating factor (GM-CSF) stimulates proliferation of hematopoietic cells of the macrophage and granulocyte lineages and is used clinically to treat neutropenia and other myeloid disorders. Because of its short circulating half-life, GM-CSF is administered to patients...