Xerogel Optical Sensor Films for Quantitative Detection of Nitroxyl

• Published in: Analytical chemistry (Washington) Vol. 80; no. 4; pp. 1247 - 1254
• Main Authors: Dobmeier, Kevin P, Riccio, Daniel A, Schoenfisch, Mark H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.02.2008
• Subjects: Acids; Analytical chemistry; Biosensing Techniques - methods; Chemistry; Exact sciences and technology; Gels - chemistry; General, instrumentation; Hydrogen-Ion Concentration; Kinetics; Manganese - chemistry; Manganese compounds; Neuroprotective Agents - analysis; Nitric oxide; Nitrogen Oxides - analysis; Optics and Photonics; Organometallic Compounds - chemistry; Organosilicon Compounds - chemistry; Porphyrins - chemistry; Sensitivity and Specificity; Sensors; Silicon Dioxide - chemistry; Nitroxyl; Microtiter plate; Optical sensor; Selectivity; Permeability; Chemical sensor; Manganese III; Sensitivity; Sodium; Efficiency; Sol gel process; Xerogel; Monitoring; Quantitative analysis
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac702024t

Xerogel sensing films synthesized via sol−gel chemistry were used to fabricate optical nitroxyl (HNO) sensors. Selective detection of HNO in solution was achieved by monitoring the rates of manganese(III) meso-tetrakis(4-sulfonatophenyl) porphyrinate (MnIIITPPS) reductive nitrosylation in the anaerobic interior of aminoalkoxysilane-derived xerogel films. Nitroxyl permeability in sensor films deposited in round-bottom 96-well plates was enhanced via incorporation of trimethoxysilyl-terminated poly(amidoamine-organosilicon) dendrimers in the xerogel network. The selectivity of MnIIITPPS for HNO, the overall sensitivity, and the working dynamic range of the resulting sensors were characterized. The HNO-sensing microtiter plates were used to quantify pH-dependent HNO generation by the recently described HNO-donor sodium 1-(isopropylamino)diazene-1-ium-1,2-diolate (IPA/NO), and compare HNO production efficiency between IPA/NO and Angeli's salt, a traditional HNO-donor.