Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures

• Published in: Biochemistry (Easton) Vol. 20; no. 16; pp. 4667 - 4676
• Main Authors: Gekko, Kunihiko, Timasheff, Serge N
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.08.1981
• Subjects: Densitometry - methods; Drug Stability; Glycerol; Hydrogen-Ion Concentration; Mathematics; Protein Conformation; Proteins; Solvents; stability; Thermodynamics; Water

A densimetric investigation of the interactions between solvent components in glycerol-water mixtures (between 10 and 40 vol % glycerol) and seven proteins have been carried out in the acid pH region. All the proteins were found to be preferentially hydrated at all conditions used, i.e., addition of...