Variability Analysis of Human Plasma and Cerebral Spinal Fluid Reveals Statistical Significance of Changes in Mass Spectrometry-Based Metabolomics Data

• Published in: Analytical chemistry (Washington) Vol. 81; no. 20; pp. 8538 - 8544
• Main Authors: Crews, Bridgit, Wikoff, William R, Patti, Gary J, Woo, Hin-Koon, Kalisiak, Ewa, Heideker, Johanna, Siuzdak, Gary
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.10.2009
• Subjects: Analysis of Variance; Analytical chemistry; Biological analysis; Biological variation; Biomarkers - blood; Biomarkers - metabolism; Blood Chemical Analysis - methods; Cerebrospinal Fluid - chemistry; Cerebrospinal Fluid - metabolism; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, Liquid; Coefficient of variation; Comparative analysis; Databases, Factual; Exact sciences and technology; Humans; Liquid chromatography; Mass spectra; Mass Spectrometry; Metabolites; Metabolomics; Nonlinear Dynamics; Other chromatographic methods; Plasma; Principal Component Analysis; Reproducibility of Results; Software; Spectrometric and optical methods; Human; Metabolomics; Peak resolution; Metabolite; Coupled method; Biological marker; Liquid chromatography; Retention time; Reproducibility; Mass spectrometry MS/MS; Robustness; Mass spectrometry; Variation coefficient

Analytical and biological variability are issues of central importance to human metabolomics studies. Here both types of variation are examined in human plasma and cerebrospinal fluid (CSF) using a global liquid chromatography/mass spectrometry (LC/MS) metabolomics strategy. The platform shows small...