Combination of High-Resolution Multistage Ion Mobility and Tandem MS with High Energy of Activation to Resolve the Structure of Complex Chemoenzymatically Synthesized Glycans

• Published in: Analytical chemistry (Washington) Vol. 94; no. 4; pp. 2279 - 2287
• Main Authors: Ropartz, David, Fanuel, Mathieu, Ollivier, Simon, Lissarrague, Adrien, Benkoulouche, Mounir, Mulard, Laurence A, André, Isabelle, Guieysse, David, Rogniaux, Hélène
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.02.2022
• Subjects: Activation energy; analytical chemistry; Biochemistry, Molecular Biology; Biological activity; Biomolecules; Carbohydrates; Charge transfer; Chemistry; Complexity; dissociation; energy; Energy charge; Energy of dissociation; Food engineering; glucosylation; Glycan; Ionic mobility; Isomerism; Isomers; Life Sciences; Mass spectrometry; Mass spectroscopy; Microorganisms; Mobility; Monosaccharides; Oligosaccharides - chemistry; Polysaccharides; Polysaccharides - chemistry; positional isomers; Reaction products; Resolution; Shigella flexneri; Structural analysis; Substrates; Sucrose; Tandem Mass Spectrometry

Carbohydrates, in particular microbial glycans, are highly structurally diverse biomolecules, the recognition of which governs numerous biological processes. Of special interest, glycans of known monosaccharide composition feature multiple possible isomers, differentiated by the anomerism and positi...