Genome-Wide Essentiality Analysis of Mycobacterium abscessus by Saturated Transposon Mutagenesis and Deep Sequencing

• Published in: mBio Vol. 12; no. 3; p. e0104921
• Main Authors: Rifat, Dalin, Chen, Liang, Kreiswirth, Barry N, Nuermberger, Eric L
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 29.06.2021
• Subjects: DNA Transposable Elements; Genes, Essential; Genome, Bacterial; Genomics - methods; High-Throughput Nucleotide Sequencing - methods; Humans; Mutagenesis, Insertional; Mycobacterium abscessus - genetics; Mycobacterium abscessus - pathogenicity; Mycobacterium tuberculosis - genetics; Research Article; transposon; mycobacterium; genetics; deep sequencing; gene disruption; transposon sequencing (Tn-Seq); genomics; Himar1 mutagenesis; essentiality; essential gene; Mycobacterium abscessus

Mycobacterium abscessus is an emerging opportunistic human pathogen that naturally resists most major classes of antibiotics, making infections difficult to treat. Thus far, little is known about physiology, pathogenesis, and drug resistance. Genome-wide analyses have comprehensively catalogued gene...