Enhanced Virus Detection and Metagenomic Sequencing in Patients with Meningitis and Encephalitis

• Published in: mBio Vol. 12; no. 4; p. e0114321
• Main Authors: Piantadosi, Anne, Mukerji, Shibani S., Ye, Simon, Leone, Michael J., Freimark, Lisa M., Park, Daniel, Adams, Gordon, Lemieux, Jacob, Kanjilal, Sanjat, Solomon, Isaac H., Ahmed, Asim A., Goldstein, Robert, Ganesh, Vijay, Ostrem, Bridget, Cummins, Kaelyn C., Thon, Jesse M., Kinsella, Cormac M., Rosenberg, Eric, Frosch, Matthew P., Goldberg, Marcia B., Cho, Tracey A., Sabeti, Pardis
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 31.08.2021
• Subjects: Adult; Aged; Central Nervous System Viral Diseases - cerebrospinal fluid; Central Nervous System Viral Diseases - diagnosis; Central Nervous System Viral Diseases - virology; encephalitis; Encephalitis - cerebrospinal fluid; Encephalitis - diagnosis; Encephalitis - virology; Female; High-Throughput Nucleotide Sequencing - methods; Humans; hybrid capture; Male; meningitis; Meningitis - cerebrospinal fluid; Meningitis - diagnosis; Meningitis - virology; Metagenome; metagenomic sequencing; Metagenomics - methods; Middle Aged; next-generation sequencing (NGS); Prospective Studies; Research Article; Virology; virus; Viruses - classification; Viruses - genetics; Viruses - isolation & purification; Viruses - pathogenicity; metagenomic sequencing; hybrid capture; encephalitis; next-generation sequencing (NGS); meningitis; methylated DNA depletion; virus

Meningitis and encephalitis are leading global causes of central nervous system (CNS) disability and mortality. Current diagnostic workflows remain inefficient, requiring costly pathogen-specific assays and sometimes invasive surgical procedures. Meningitis and encephalitis are leading causes of cen...