MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells

• Published in: mBio Vol. 12; no. 5; p. e0148421
• Main Authors: Villalón-Letelier, Fernando, Brooks, Andrew G, Londrigan, Sarah L, Reading, Patrick C
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 26.10.2021
• Subjects: Animals; Dogs; Down-Regulation; Gene Expression; HEK293 Cells; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Host-Microbial Interactions; Humans; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - pathogenicity; Influenza A Virus, H3N2 Subtype - genetics; Influenza A Virus, H3N2 Subtype - pathogenicity; Madin Darby Canine Kidney Cells; Research Article; Ubiquitin-Protein Ligases - genetics; Virus Replication - genetics; influenza; glycoproteins; RNA virus; ubiquitination

Membrane-associated RING-CH8 (MARCH8) impairs the cell surface expression of envelope glycoproteins from different viruses, reducing their incorporation into virions. Using stable cell lines with inducible MARCH8 expression, we show that MARCH8 did not alter susceptibility to influenza A virus (IAV)...