Microbiome Analysis for Wastewater Surveillance during COVID-19

• Published in: mBio Vol. 13; no. 4; p. e0059122
• Main Authors: Brumfield, Kyle D., Leddy, Menu, Usmani, Moiz, Cotruvo, Joseph A., Tien, Ching-Tzone, Dorsey, Suzanne, Graubics, Karlis, Fanelli, Brian, Zhou, Isaac, Registe, Nathaniel, Dadlani, Manoj, Wimalarante, Malinda, Jinasena, Dilini, Abayagunawardena, Rushan, Withanachchi, Chiran, Huq, Anwar, Jutla, Antarpreet, Colwell, Rita R.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 30.08.2022
• Subjects: COVID-19; COVID-19 - epidemiology; COVID-19 Testing; Editor's Pick; Genomics and Proteomics; Humans; microbiome; Microbiota; Research Article; RNA, Viral - analysis; RNA, Viral - genetics; SARS-CoV-2; SARS-CoV-2 - genetics; Wastewater; wastewater monitoring; Wastewater-Based Epidemiological Monitoring; wastewater-based epidemiology; RNA sequencing; risk assessment; wastewater; wastewater monitoring; wastewater surveillance; environmental risk; shotgun sequencing; wastewater-based epidemiology; microbiome; COVID-19; SARS-CoV-2; metatranscriptomics; metagenomics; whole metagenome sequencing; RT-qPCR; DNA sequencing

Traditionally, testing for COVID-19 is done by detecting SARS-CoV-2 in samples collected from nasal swabs and/or saliva. However, SARS-CoV-2 can also be detected in feces of infected individuals. Wastewater surveillance (WS), when coupled with advanced molecular techniques, offers near real-time mon...