Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Ligands

• Published in: Journal of the American Chemical Society Vol. 141; no. 50; pp. 19585 - 19588
• Main Authors: Goldberg, Nathaniel W, Knight, Anders M, Zhang, Ruijie K, Arnold, Frances H
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 18.12.2019
• Subjects: Alkenes - chemistry; aminocyclopropanecarboxylate oxidase; Aziridines - chemistry; Biocatalysis; catalytic activity; chemical bonding; directed evolution; Directed Molecular Evolution; enzyme activity; iron; Ligands; Models, Molecular; Nonheme Iron Proteins - chemistry; Nonheme Iron Proteins - genetics; Nonheme Iron Proteins - metabolism; olefin; Protein Conformation; Pseudomonas - enzymology; Pseudomonas syringae pv. savastanoi

Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that Pseudomonas savastanoi ethylene-forming enzyme, a non-heme iron enzyme, can catalyze olefin aziridination and nitrene C–H insertion, and that these activities can be improved by directed evolution...