Comparative Proteomic Profiling of Unannotated Microproteins and Alternative Proteins in Human Cell Lines

• Published in: Journal of proteome research Vol. 19; no. 8; pp. 3418 - 3426
• Main Authors: Cao, Xiongwen, Khitun, Alexandra, Na, Zhenkun, Dumitrescu, Daniel G, Kubica, Marcelina, Olatunji, Elizabeth, Slavoff, Sarah A
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.08.2020
• Subjects: Cell Line; complementary DNA; gene expression regulation; Humans; Mass Spectrometry; neoplasm cells; Open Reading Frames; polypeptides; proteins; proteome; Proteome - genetics; Proteomics; ribosomes; translation (genetics); comparative proteomics; unannotated protein; proteogenomics; microprotein; alternative protein

Ribosome profiling and mass spectrometry have revealed thousands of small and alternative open reading frames (sm/alt-ORFs) that are translated into polypeptides variously termed as microproteins and alt-proteins in mammalian cells. Some micro-/alt-proteins exhibit stress-, cell-type-, and/or tissue...