High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes

The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro...

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Published inEnvironmental science & technology Vol. 58; no. 4; pp. 2027 - 2037
Main Authors Rogers, Jesse D., Leusch, Frederic D.L., Chambers, Bryant, Daniels, Kevin D., Everett, Logan J., Judson, Richard, Maruya, Keith, Mehinto, Alvine C., Neale, Peta A., Paul-Friedman, Katie, Thomas, Russell, Snyder, Shane A., Harrill, Joshua
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 30.01.2024
American Chemical Society (ACS)
Subjects
Bioassays
Biological activity
Biological Assay
Breast carcinoma
Cell proliferation
Chemical pollution
Contaminants
Cost analysis
Drinking water
Ecological risk assessment
Engineering
Environmental Monitoring
Environmental Sciences & Ecology
Gene expression
Gene Expression Profiling
Humans
Industrial pollution
Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants
Quality assessment
Reclaimed water
Surface water
Transcriptomics
Water monitoring
Water Pollutants, Chemical - analysis
Water Purification
Water Quality
Water quality management
Water supply
Water treatment
Xenoestrogens
differentially expressed genes
recycled water
MCF7
water cycle
water quality
high throughput transcriptomics
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Abstract The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.
AbstractList The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.
The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.
The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.
Not provided.
The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.
Author Thomas, Russell
Daniels, Kevin D.
Harrill, Joshua
Leusch, Frederic D.L.
Rogers, Jesse D.
Chambers, Bryant
Snyder, Shane A.
Mehinto, Alvine C.
Maruya, Keith
Neale, Peta A.
Paul-Friedman, Katie
Everett, Logan J.
Judson, Richard
AuthorAffiliation Stantec Inc
Oak Ridge Institute for Science and Education
Australian Rivers Institute, School of Environment and Science
Center for Computational Toxicology and Exposure, Office of Research and Development
Nanyang Environment & Water Research Institute (NEWRI)
Griffith University
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Snippet The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and...
Not provided.
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osti
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StartPage 2027
SubjectTerms Bioassays
Biological activity
Biological Assay
Breast carcinoma
Cell proliferation
Chemical pollution
Contaminants
Cost analysis
Drinking water
Ecological risk assessment
Engineering
Environmental Monitoring
Environmental Sciences & Ecology
Gene expression
Gene Expression Profiling
Humans
Industrial pollution
Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants
Quality assessment
Reclaimed water
Surface water
Transcriptomics
Water monitoring
Water Pollutants, Chemical - analysis
Water Purification
Water Quality
Water quality management
Water supply
Water treatment
Xenoestrogens
Title High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes
URI http://dx.doi.org/10.1021/acs.est.3c07525
https://www.ncbi.nlm.nih.gov/pubmed/38235672
https://www.proquest.com/docview/2920664872
https://www.proquest.com/docview/2916409113
https://www.osti.gov/biblio/2581448
https://pubmed.ncbi.nlm.nih.gov/PMC11003563
Volume 58
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