CRISPR/Cas9 Genome Editing To Demonstrate the Contribution of Cyp51A Gly138Ser to Azole Resistance in Aspergillus fumigatus

• Published in: Antimicrobial agents and chemotherapy Vol. 62; no. 9
• Main Authors: Umeyama, Takashi, Hayashi, Yuta, Shimosaka, Hisaki, Inukai, Tatsuya, Yamagoe, Satoshi, Takatsuka, Shogo, Hoshino, Yasutaka, Nagi, Minoru, Nakamura, Shigeki, Kamei, Katsuhiko, Ogawa, Kenji, Miyazaki, Yoshitsugu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.09.2018
• Subjects: Antifungal Agents; Aspergillosis; Aspergillus fumigatus; Cytochrome P-450 Enzyme System; Drug Resistance, Fungal; Fungal Proteins; Gene Editing; Mechanisms of Resistance; Voriconazole; CRISPR; genome editing; Cyp51A; Cas9; azole drugs; antifungal resistance

A pan-azole-resistant Aspergillus fumigatus strain with the cyp51A mutations Gly138Ser and Asn248Lys was isolated from a patient receiving long-term voriconazole treatment. PCR fragments containing cyp51A with the mutations were introduced along with the Cas9 protein and single guide RNA into the az...