Multiplex SARS-CoV-2 Genotyping Reverse Transcriptase PCR for Population-Level Variant Screening and Epidemiologic Surveillance

• Published in: Journal of clinical microbiology Vol. 59; no. 8; p. e0085921
• Main Authors: Wang, Hannah, Miller, Jacob A., Verghese, Michelle, Sibai, Mamdouh, Solis, Daniel, Mfuh, Kenji O., Jiang, Becky, Iwai, Naomi, Mar, Marilyn, Huang, ChunHong, Yamamoto, Fumiko, Sahoo, Malaya K., Zehnder, James, Pinsky, Benjamin A.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 19.07.2021
• Subjects: COVID-19; Epidemiological Monitoring; Genotype; Humans; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2; Virology; COVID-19; variant; SARS-CoV-2; RT-qPCR; genotyping

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with concerning phenotypic mutations is of public health interest. Genomic surveillance is an important tool for a pandemic response, but many laboratories do not have the resources to support population-level seq...