Spectrum of DNA-platinum adduct recognition by prokaryotic and eukaryotic DNA-dependent RNA polymerases

• Published in: Biochemistry (Easton) Vol. 32; no. 33; pp. 8582 - 8588
• Main Authors: Corda, Yves, Job, Claudette, Anin, Marie France, Leng, Marc, Job, Dominique
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 24.08.1993
• Subjects: Antineoplastic agents; Base Sequence; Biological and medical sciences; DNA - metabolism; DNA-Directed RNA Polymerases - metabolism; Escherichia coli - enzymology; General aspects; Kinetics; Medical sciences; Molecular Sequence Data; Oligodeoxyribonucleotides - chemical synthesis; Oligodeoxyribonucleotides - metabolism; Pharmacology. Drug treatments; Platinum - metabolism; RNA - biosynthesis; Structure-Activity Relationship; Templates, Genetic; Transcription, Genetic; Triticum - enzymology; Antineoplastic agent; Transcription; Enzyme; DNA; RNA polymerase; Mechanism of action; In vitro; Molecular adduct; Platinum II Complexes

Double-stranded DNA oligomers were constructed to evaluate the effect of bifunctional and monofunctional platinum(II) complexes at the level of DNA transcription. They contained a single lesion, which is either a cis-[Pt(NH3)2(d(GpTpG))] intrastrand cross-link, a trans-[Pt(NH3)2(d(GpTpG))] intrastra...