Picomolar Assay of Native Proteins by Capillary Electrophoresis Precolumn Labeling, Submicellar Separation, and Laser-Induced Fluorescence Detection

• Published in: Analytical chemistry (Washington) Vol. 69; no. 15; pp. 3015 - 3021
• Main Authors: Pinto, Devanand M, Arriaga, Edgar A, Craig, Doug, Angelova, Jordanka, Sharma, Neepun, Ahmadzadeh, Hossein, Dovichi, Norman J, Boulet, Camille A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.08.1997
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; fluorescence; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; labeling; monomers; peptides and proteins; Proteins; sodium dodecyl sulfate; Trace analysis; Proteins; Gel electrophoresis; Lysine; Fluorescent labelling; Analytical method; Capillary tube

We report a method for the assay of proteins at concentrations lower than 10-10 M with as little as 200 amol of protein. High sensitivity is accomplished by derivatizing the ε-amino group of the protein's lysine residues with the fluorogenic dye 5-furoylquinoline-3-carboxaldehyde and use of a s...