Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy

• Published in: The journal of physical chemistry. B Vol. 122; no. 7; pp. 2073 - 2081
• Main Authors: Viger-Gravel, Jasmine, Schantz, Anna, Pinon, Arthur C, Rossini, Aaron J, Schantz, Staffan, Emsley, Lyndon
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.02.2018
• Subjects: cholesterol; drugs; encapsulation; ethanolamine; messenger RNA; mixing; nanoparticles; nuclear magnetic resonance spectroscopy; phospholipids; phosphorus; physical chemistry; polyethylene glycol; small interfering RNA; stable isotopes

Here, we show how dynamic nuclear polarization (DNP) NMR spectroscopy experiments permit the atomic level structural characterization of loaded and empty lipid nanoparticles (LNPs). The LNPs used here were synthesized by the microfluidic mixing technique and are composed of ionizable cationic lipid...