Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer

• Published in: Annual review of biochemistry Vol. 80; p. 357
• Main Author: Miyawaki, Atsushi
• Format: Journal Article
• Language: English
• Published: United States 07.07.2011
• Subjects: Animals; Cell Physiological Phenomena; Fluorescence Resonance Energy Transfer - methods; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Microscopy, Fluorescence - methods; Models, Molecular; Protein Conformation; Proteins

Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization...