Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer
Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization...
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| Published in | Annual review of biochemistry Vol. 80; p. 357 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
United States
07.07.2011
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| Subjects | |
| Online Access | Get more information |
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| Abstract | Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization of their specific features. Here I provide theoretical and practical comparisons of bimolecular and unimolecular FRET constructs, intensity-based and lifetime-based FRET measurements, FRET imaging using live- and fixed-cell samples, green fluorescent protein-based and chemical fluorophore-based FRET, and FRET efficiency and indices. The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed. |
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| AbstractList | Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization of their specific features. Here I provide theoretical and practical comparisons of bimolecular and unimolecular FRET constructs, intensity-based and lifetime-based FRET measurements, FRET imaging using live- and fixed-cell samples, green fluorescent protein-based and chemical fluorophore-based FRET, and FRET efficiency and indices. The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed. |
| Author | Miyawaki, Atsushi |
| Author_xml | – sequence: 1 givenname: Atsushi surname: Miyawaki fullname: Miyawaki, Atsushi email: matsushi@brain.riken.jp organization: Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako-city, Saitama 351-0198, Japan. matsushi@brain.riken.jp |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21529159$$D View this record in MEDLINE/PubMed |
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| Snippet | Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the... |
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| SubjectTerms | Animals Cell Physiological Phenomena Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Microscopy, Fluorescence - methods Models, Molecular Protein Conformation Proteins |
| Title | Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer |
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