Three-Dimensional DNA Nanomachine Biosensor by Integrating DNA Walker and Rolling Machine Cascade Amplification for Ultrasensitive Detection of Cancer-Related Gene

• Published in: Analytical chemistry (Washington) Vol. 92; no. 16; pp. 11111 - 11118
• Main Authors: Wu, Na, Wang, Kun, Wang, Yi-Ting, Chen, Ming-Li, Chen, Xu-Wei, Yang, Ting, Wang, Jian-Hua
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 18.08.2020
• Subjects: Amplification; Analytical chemistry; Biosensing Techniques - methods; Biosensors; blood; Chemistry; Deoxyribonucleic acid; detection limit; DNA; DNA - blood; DNA - chemistry; DNA - genetics; Exonuclease; genes; Genes, p53; Gold; Gold - chemistry; Humans; Inverted Repeat Sequences; Limit of Detection; Magnetic Phenomena; magnetism; mass spectrometry; Metal Nanoparticles - chemistry; Nanoclusters; nanogold; Nanoparticles; Nucleic Acid Amplification Techniques - methods; Nucleic Acid Hybridization; p53 Protein; Silver; Silver - chemistry; Spectrometry; Tumor Suppressor Protein p53 - genetics; Walking

Stochastic DNA walkers capable of traversing on three-dimensional (3D) tracks have received great deal of attention. However, DNA walker-based biosensors exhibit limited amplification efficiency because of their slow walking kinetics and low processivity. Herein, by taking advantage of the high proc...