Aptamer Affinity Maturation by Resampling and Microarray Selection
Aptamers have significant potential as affinity reagents, but better approaches are critically needed to discover higher affinity nucleic acids to widen the scope for their diagnostic, therapeutic, and proteomic application. Here, we report aptamer affinity maturation, a novel aptamer enhancement te...
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| Published in | Analytical chemistry (Washington) Vol. 88; no. 14; pp. 6981 - 6985 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Chemical Society
19.07.2016
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Aptamers have significant potential as affinity reagents, but better approaches are critically needed to discover higher affinity nucleic acids to widen the scope for their diagnostic, therapeutic, and proteomic application. Here, we report aptamer affinity maturation, a novel aptamer enhancement technique, which combines bioinformatic resampling of aptamer sequence data and microarray selection to navigate the combinatorial chemistry binding landscape. Aptamer affinity maturation is shown to improve aptamer affinity by an order of magnitude in a single round. The novel aptamers exhibited significant adaptation, the complexity of which precludes discovery by other microarray based methods. Honing aptamer sequences using aptamer affinity maturation could help optimize a next generation of nucleic acid affinity reagents. |
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| AbstractList | Aptamers have significant potential as affinity reagents, but better approaches are critically needed to discover higher affinity nucleic acids to widen the scope for their diagnostic, therapeutic, and proteomic application. Here, we report aptamer affinity maturation, a novel aptamer enhancement technique, which combines bioinformatic resampling of aptamer sequence data and microarray selection to navigate the combinatorial chemistry binding landscape. Aptamer affinity maturation is shown to improve aptamer affinity by an order of magnitude in a single round. The novel aptamers exhibited significant adaptation, the complexity of which precludes discovery by other microarray based methods. Honing aptamer sequences using aptamer affinity maturation could help optimize a next generation of nucleic acid affinity reagents. Aptamers have significant potential as affinity reagents, but better approaches are critically needed to discover higher affinity nucleic acids to widen the scope for their diagnostic, therapeutic, and proteomic application. Here, we report aptamer affinity maturation, a novel aptamer enhancement technique, which combines bioinformatic resampling of aptamer sequence data and microarray selection to navigate the combinatorial chemistry binding landscape. Aptamer affinity maturation is shown to improve aptamer affinity by an order of magnitude in a single round. The novel aptamers exhibited significant adaptation, the complexity of which precludes discovery by other microarray based methods. Honing aptamer sequences using aptamer affinity maturation could help optimize a next generation of nucleic acid affinity reagents.Aptamers have significant potential as affinity reagents, but better approaches are critically needed to discover higher affinity nucleic acids to widen the scope for their diagnostic, therapeutic, and proteomic application. Here, we report aptamer affinity maturation, a novel aptamer enhancement technique, which combines bioinformatic resampling of aptamer sequence data and microarray selection to navigate the combinatorial chemistry binding landscape. Aptamer affinity maturation is shown to improve aptamer affinity by an order of magnitude in a single round. The novel aptamers exhibited significant adaptation, the complexity of which precludes discovery by other microarray based methods. Honing aptamer sequences using aptamer affinity maturation could help optimize a next generation of nucleic acid affinity reagents. |
| Author | Tanner, Julian A Cheung, Yee-Wai Fraser, Lewis A Kinghorn, Andrew B Shiu, Simon Chi-Chin Liang, Shaolin Dirkzwager, Roderick M Tang, Marco S. L |
| AuthorAffiliation | The University of Hong Kong School of Biomedical Sciences, Li Ka Shing Faculty of Medicine |
| AuthorAffiliation_xml | – name: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine – name: The University of Hong Kong |
| Author_xml | – sequence: 1 givenname: Andrew B surname: Kinghorn fullname: Kinghorn, Andrew B – sequence: 2 givenname: Roderick M surname: Dirkzwager fullname: Dirkzwager, Roderick M – sequence: 3 givenname: Shaolin surname: Liang fullname: Liang, Shaolin – sequence: 4 givenname: Yee-Wai surname: Cheung fullname: Cheung, Yee-Wai – sequence: 5 givenname: Lewis A surname: Fraser fullname: Fraser, Lewis A – sequence: 6 givenname: Simon Chi-Chin surname: Shiu fullname: Shiu, Simon Chi-Chin – sequence: 7 givenname: Marco S. L surname: Tang fullname: Tang, Marco S. L – sequence: 8 givenname: Julian A surname: Tanner fullname: Tanner, Julian A email: jatanner@hku.hk |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27346322$$D View this record in MEDLINE/PubMed |
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| DOI | 10.1021/acs.analchem.6b01635 |
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| Title | Aptamer Affinity Maturation by Resampling and Microarray Selection |
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