Integrated Proteome Analysis Device for Fast Single-Cell Protein Profiling

• Published in: Analytical chemistry (Washington) Vol. 90; no. 23; pp. 14003 - 14010
• Main Authors: Shao, Xi, Wang, Xuantang, Guan, Sheng, Lin, Haizhu, Yan, Guoquan, Gao, Mingxia, Deng, Chunhui, Zhang, Xiangmin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.12.2018
• Subjects: Analytical chemistry; Blood vessels; Cell cycle; Cellular biology; Chemistry; detection limit; Digestion; Dilution; Elution; Heterogeneity; High temperature; histones; human cell lines; Lysis; Peptides; protein composition; protein depletion; Protein expression; Proteins; proteome; Proteomes; Single-cell protein; temperature; Ultrasonic imaging; ultrasonic treatment

In our previous work, we have demonstrated an integrated proteome analysis device (iPAD-100) to analyze proteomes from 100 cells. In this work, for the first time, a novel integrated device for single-cell analysis (iPAD-1) was developed to profile proteins in a single cell within 1 h. In the iPAD-1...