Silver Nanoclusters-Based Fluorescence Assay of Protein Kinase Activity and Inhibition

• Published in: Analytical chemistry (Washington) Vol. 87; no. 1; pp. 693 - 698
• Main Authors: Shen, Congcong, Xia, Xiaodong, Hu, Shengqiang, Yang, Minghui, Wang, Jianxiu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.01.2015
• Subjects: Adenosine diphosphate; Adenosine triphosphatase; adenosine triphosphate; Adenosine Triphosphate - metabolism; Assaying; ATP; Biological Assay; cAMP-dependent protein kinase; Cyclic AMP-Dependent Protein Kinases - chemistry; Cyclic AMP-Dependent Protein Kinases - metabolism; Enzyme Inhibitors - pharmacology; Fluorescence; HeLa Cells; Humans; hydrolysis; Inhibition; Inhibitors; Kinases; Metal Nanoparticles - chemistry; monitoring; Monitoring methods; nanoparticles; Nanostructure; Nanostructures - chemistry; oligonucleotides; Peptides; Phosphorylation; Silver; Silver - chemistry; Spectrometry, Fluorescence

A simple and sensitive fluorescence method for monitoring the activity and inhibition of protein kinase (PKA) has been developed using polycytosine oligonucleotide (dC12)-templated silver nanoclusters (Ag NCs). Adenosine-5′-triphosphate (ATP) was found to enhance the fluorescence of Ag NCs, while th...