Rapid DNA Chemical Ligation for Amplification of RNA and DNA Signal

• Published in: Bioconjugate chemistry Vol. 19; no. 1; pp. 327 - 333
• Main Authors: Abe, Hiroshi, Kondo, Yuko, Jinmei, Hiroshi, Abe, Naoko, Furukawa, Kazuhiro, Uchiyama, Atsushi, Tsuneda, Satoshi, Aikawa, Kyoko, Matsumoto, Isamu, Ito, Yoshihiro
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.01.2008
• Subjects: Base Pair Mismatch; Base Sequence; Biochemistry; Deoxyribonucleic acid; DNA; DNA - analysis; DNA - chemistry; DNA - genetics; Enzymes; Gene amplification; Hot Temperature; Iodoacetates - chemical synthesis; Iodoacetates - chemistry; Nucleic Acid Amplification Techniques - methods; Nucleic Acid Denaturation; Organothiophosphorus Compounds - chemical synthesis; Organothiophosphorus Compounds - chemistry; Ribonucleic acid; RNA; RNA - analysis; RNA - chemistry; RNA - genetics

Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate−iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is...