Dual-Signal Readout Nanospheres for Rapid Point-of-Care Detection of Ebola Virus Glycoprotein

Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of Ebola virus (EBOV) glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a report...

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Published in	Analytical chemistry (Washington) Vol. 89; no. 24; pp. 13105 - 13111
Main Authors	Hu, Jiao, Jiang, Yong-Zhong, Wu, Ling-Ling, Wu, Zhen, Bi, Yuhai, Wong, Gary, Qiu, Xiangguo, Chen, Jianjun, Pang, Dai-Wen, Zhang, Zhi-Ling
Format	Journal Article
Language	English
Published	United States American Chemical Society 19.12.2017
Subjects	antibodies Assaying Biotin Chemistry Colorimetry detection limit Drinking water early diagnosis Ebola virus Ebolavirus Ebolavirus - chemistry Fluorescence Glycoproteins Glycoproteins - analysis Gold Gold - chemistry Humans nanogold Nanoparticles Nanospheres Nanospheres - chemistry pathogens Point-of-Care Systems probability Quantum dots rapid methods Streptavidin tap water Urine Visual signals Water analysis Water sampling
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Abstract	Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of Ebola virus (EBOV) glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target and act as signal enhancer. After the target was labeled by the Ab–RNs@Au–SA and captured on the test line, biotin-modified RNs@Au was used to amplify the dual signal by the reaction of SA with biotin. The assay enables naked-eye detection of 2 ng/mL glycoprotein within 20 min, and the quantitative detection limit is 0.18 ng/mL. Additionally, the assay has been successfully tested in field work for detecting EBOV in spiked urine, plasma, and tap water samples and is thus a promising candidate for early diagnosis of suspect infections in EBOV-stricken areas.
AbstractList	Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of Ebola virus (EBOV) glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target and act as signal enhancer. After the target was labeled by the Ab–RNs@Au–SA and captured on the test line, biotin-modified RNs@Au was used to amplify the dual signal by the reaction of SA with biotin. The assay enables naked-eye detection of 2 ng/mL glycoprotein within 20 min, and the quantitative detection limit is 0.18 ng/mL. Additionally, the assay has been successfully tested in field work for detecting EBOV in spiked urine, plasma, and tap water samples and is thus a promising candidate for early diagnosis of suspect infections in EBOV-stricken areas. Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of Ebola virus (EBOV) glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target and act as signal enhancer. After the target was labeled by the Ab-RNs@Au-SA and captured on the test line, biotin-modified RNs@Au was used to amplify the dual signal by the reaction of SA with biotin. The assay enables naked-eye detection of 2 ng/mL glycoprotein within 20 min, and the quantitative detection limit is 0.18 ng/mL. Additionally, the assay has been successfully tested in field work for detecting EBOV in spiked urine, plasma, and tap water samples and is thus a promising candidate for early diagnosis of suspect infections in EBOV-stricken areas.Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of Ebola virus (EBOV) glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target and act as signal enhancer. After the target was labeled by the Ab-RNs@Au-SA and captured on the test line, biotin-modified RNs@Au was used to amplify the dual signal by the reaction of SA with biotin. The assay enables naked-eye detection of 2 ng/mL glycoprotein within 20 min, and the quantitative detection limit is 0.18 ng/mL. Additionally, the assay has been successfully tested in field work for detecting EBOV in spiked urine, plasma, and tap water samples and is thus a promising candidate for early diagnosis of suspect infections in EBOV-stricken areas.
Author	Jiang, Yong-Zhong Bi, Yuhai Wu, Ling-Ling Qiu, Xiangguo Hu, Jiao Pang, Dai-Wen Wu, Zhen Wong, Gary Chen, Jianjun Zhang, Zhi-Ling
AuthorAffiliation	Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology Chinese Academy of Sciences Center for Influenza Research and Early Warning Shenzhen Third People’s Hospital Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Department of Medical Microbiology Institute of Microbiology Chinese Academy of Sciences Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease
AuthorAffiliation_xml	– name: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – name: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology – name: Shenzhen Third People’s Hospital – name: Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease – name: Chinese Academy of Sciences Center for Influenza Research and Early Warning – name: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Department of Medical Microbiology – name: Institute of Microbiology – name: Chinese Academy of Sciences Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology
Author_xml	– sequence: 1 givenname: Jiao surname: Hu fullname: Hu, Jiao organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – sequence: 2 givenname: Yong-Zhong surname: Jiang fullname: Jiang, Yong-Zhong organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – sequence: 3 givenname: Ling-Ling surname: Wu fullname: Wu, Ling-Ling organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – sequence: 4 givenname: Zhen surname: Wu fullname: Wu, Zhen organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – sequence: 5 givenname: Yuhai surname: Bi fullname: Bi, Yuhai organization: Chinese Academy of Sciences Center for Influenza Research and Early Warning – sequence: 6 givenname: Gary surname: Wong fullname: Wong, Gary organization: Chinese Academy of Sciences Center for Influenza Research and Early Warning – sequence: 7 givenname: Xiangguo surname: Qiu fullname: Qiu, Xiangguo organization: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Department of Medical Microbiology – sequence: 8 givenname: Jianjun surname: Chen fullname: Chen, Jianjun email: chenjj@wh.iov.cn organization: Chinese Academy of Sciences Center for Influenza Research and Early Warning – sequence: 9 givenname: Dai-Wen orcidid: 0000-0002-7017-5725 surname: Pang fullname: Pang, Dai-Wen organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology – sequence: 10 givenname: Zhi-Ling orcidid: 0000-0001-7807-2264 surname: Zhang fullname: Zhang, Zhi-Ling email: zlzhang@whu.edu.cn organization: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology
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Snippet	Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and...
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SubjectTerms	antibodies Assaying Biotin Chemistry Colorimetry detection limit Drinking water early diagnosis Ebola virus Ebolavirus Ebolavirus - chemistry Fluorescence Glycoproteins Glycoproteins - analysis Gold Gold - chemistry Humans nanogold Nanoparticles Nanospheres Nanospheres - chemistry pathogens Point-of-Care Systems probability Quantum dots rapid methods Streptavidin tap water Urine Visual signals Water analysis Water sampling
Title	Dual-Signal Readout Nanospheres for Rapid Point-of-Care Detection of Ebola Virus Glycoprotein
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