Stable Isotope Sequential Derivatization for Linkage-Specific Analysis of Sialylated N‑Glycan Isomers by MS

• Published in: Analytical chemistry (Washington) Vol. 91; no. 24; pp. 15993 - 16001
• Main Authors: Peng, Ye, Wang, Limeng, Zhang, Ying, Bao, Huimin, Lu, Haojie
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.12.2019
• Subjects: Biological activity; blood serum; Chemistry; chromatography; derivatization; Glycan; Ionization; Ions; Isomers; Linkage analysis; Mass spectrometry; Mass spectroscopy; N-glycans; patients; Polysaccharides; Quantitation; Selectivity; sialic acid; Sialic acids; Stable isotopes

Sialylated N-glycans play pivotal role in several important biological and pathological processes. Their sialyl-linkage isomers, mostly α-2,3- and α-2,6-linked, act differently during the cellular events and several diseases. While mass spectrometry (MS) technology is a powerful tool in N-glycome an...