Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization in Transmission Geometry
In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, the laser typically illuminates the analyte on the front side of an opaque surface (reflection geometry). Another configuration consisting of laser illumination through the sample backside (transmis...
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| Published in | Analytical chemistry (Washington) Vol. 74; no. 8; pp. 1891 - 1895 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Chemical Society
15.04.2002
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| Subjects | |
| Online Access | Get full text |
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| Abstract | In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, the laser typically illuminates the analyte on the front side of an opaque surface (reflection geometry). Another configuration consisting of laser illumination through the sample backside (transmission geometry) has been used in conventional MALDI; however, its use and the number of reports in the literature are limited. The viability of transmission geometry with AP MALDI is demonstrated here. Such a geometry is simple to implement, eliminates the restriction for a metallic sample holder, and allows for the potential analysis of samples on their native transparent surfaces, e.g., cells or tissue sections on slides. |
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| AbstractList | In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, the laser typically illuminates the analyte on the front side of an opaque surface (reflection geometry). Another configuration consisting of laser illumination through the sample backside (transmission geometry) has been used in conventional MALDI; however, its use and the number of reports in the literature are limited. The viability of transmission geometry with AP MALDI is demonstrated here. Such a geometry is simple to implement, eliminates the restriction for a metallic sample holder, and allows for the potential analysis of samples on their native transparent surfaces, e.g., cells or tissue sections on slides. In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, the laser typically illuminates the analyte on the front side of an opaque surface (reflection geometry.) The viability of transmission geometry with AP MALDI is demonstrated. |
| Author | Vertes, Akos Galicia, Marsha C Callahan, John H |
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| Cites_doi | 10.1021/ja00048a013 10.1016/S0169-4332(00)00824-2 10.1016/S1044-0305(99)00084-7 10.1021/la0016874 10.1002/(SICI)1097-0231(199610)10:13<1575::AID-RCM658>3.0.CO;2-C 10.1002/(SICI)1097-0231(199605)10:7<821::AID-RCM566>3.0.CO;2-A 10.1002/(SICI)1097-0231(199612)10:15<1873::AID-RCM719>3.0.CO;2-3 |
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| Keywords | Direct method Sensitivity Matrix assisted laser desorption ionization Transmission mode Atmospheric pressure Mass spectrometry |
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| SubjectTerms | Analytical chemistry Angiotensin I - chemistry Atmospheric Pressure Chemistry Exact sciences and technology Gas Chromatography-Mass Spectrometry - methods Geometry Lasers Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
| Title | Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization in Transmission Geometry |
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