Rapid and Highly Sensitive Method for Influenza A (H1N1) Virus Detection

• Published in: Analytical chemistry (Washington) Vol. 84; no. 9; pp. 3914 - 3920
• Main Authors: Su, Li-Chen, Chang, Chung-Ming, Tseng, Ya-Ling, Chang, Ying-Feng, Li, Ying-Chang, Chang, Yu-Sun, Chou, Chien
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2012
• Subjects: Analytical chemistry; Animals; antibodies; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; detection limit; diagnostic techniques; Equipment Design; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Humans; Influenza; Influenza A virus; Influenza A Virus, H1N1 Subtype - isolation & purification; Limit of Detection; Methods. Procedures. Technologies; nasal mucosa; Orthomyxoviridae Infections - diagnosis; Orthomyxoviridae Infections - veterinary; Pathogens; Phosphates; Plasma; Polymerase chain reaction; quantitative polymerase chain reaction; rapid methods; surface plasmon resonance; Surface Plasmon Resonance - economics; Surface Plasmon Resonance - instrumentation; Surface Plasmon Resonance - methods; Swine - virology; Time Factors; Various methods and equipments; viruses; Human; Phosphates; Plasma; Chemical analysis; Stability; Antibody; Sample; Mucosa; Surface wave; Concentration; Chemical sensor; Real time; Polymerase chain reaction; Virus; In vivo; Signal; Biosensor; Detection limit; Alternative method; Surface plasmon resonance; Diagnosis; Detection; Curve fitting

In this study, we applied the developed paired surface plasma waves biosensor (PSPWB) in a dual-channel biosensor for rapid and sensitive detection of swine-origin influenza A (H1N1) virus (S-OIV). In conjunction with the amplitude ratio of the signal and the reference channel, the stability of the...