Efficient pathway-driven scyllo -inositol production from myo -inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control

• Published in: Applied and Environmental Microbiology Vol. 90; no. 7; p. e0028124
• Main Authors: Kurashiki, Ryota, Takahashi, Masahiro, Okumura, Yuta, Ono, Tatsuya, Endo, Hirofumi, Makino, Kohei, Fukui, Kaho, Yokoyama, Kyosuke, Ishikawa, Shu, Yoshida, Ken-ichi, Ohshiro, Takashi, Suzuki, Hirokazu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 24.07.2024
• Subjects: Bacillus subtilis - enzymology; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biotechnology; Escherichia coli - genetics; Escherichia coli - metabolism; Geobacillus - enzymology; Geobacillus - genetics; Geobacillus - metabolism; Inositol - metabolism; Promoter Regions, Genetic; Sugar Alcohol Dehydrogenases - genetics; Sugar Alcohol Dehydrogenases - metabolism; iol gene; pathway engineering; bioconversion; Bacillus; Geobacillus; cell-free synthesis; enzyme cocktail

Enzyme cocktails are commonly employed for cell-free chemical synthesis; however, their preparation involves cumbersome processes. This study affirms that mesophilic enzymes in thermophilic crude extracts can function as specific catalysts at moderate temperatures, akin to enzyme cocktails. The cata...