Integrated Nanoplasmonic Sensing for Cellular Functional Immunoanalysis Using Human Blood
Localized surface plasmon resonance (LSPR) nanoplasmonic effects allow for label-free, real-time detection of biomolecule binding events on a nanostructured metallic surface with simple optics and sensing tunability. Despite numerous reports on LSPR bionanosensing in the past, no study thus far has...
Saved in:
Published in | ACS nano Vol. 8; no. 3; pp. 2667 - 2676 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
25.03.2014
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Localized surface plasmon resonance (LSPR) nanoplasmonic effects allow for label-free, real-time detection of biomolecule binding events on a nanostructured metallic surface with simple optics and sensing tunability. Despite numerous reports on LSPR bionanosensing in the past, no study thus far has applied the technique for a cytokine secretion assay using clinically relevant immune cells from human blood. Cytokine secretion assays, a technique to quantify intercellular-signaling proteins secreted by blood immune cells, allow determination of the functional response of the donor’s immune cells, thus providing valuable information about the immune status of the donor. However, implementation of LSPR bionanosensing in cellular functional immunoanalysis based on a cytokine secretion assay poses major challenges primarily owing to its limited sensitivity and a lack of sufficient sample handling capability. In this paper, we have developed a label-free LSPR biosensing technique to detect cell-secreted tumor necrosis factor (TNF)-α cytokines in clinical blood samples. Our approach integrates LSPR bionanosensors in an optofluidic platform that permits trapping and stimulation of target immune cells in a microfluidic chamber with optical access for subsequent cytokine detection. The on-chip spatial confinement of the cells is the key to rapidly increasing a cytokine concentration high enough for detection by the LSPR setup, thereby allowing the assay time and sample volume to be significantly reduced. We have successfully applied this approach first to THP-1 cells and then later to CD45 cells isolated directly from human blood. Our LSPR optofluidics device allows for detection of TNF-α secreted from cells as few as 1000, which translates into a nearly 100 times decrease in sample volume than conventional cytokine secretion assay techniques require. We achieved cellular functional immunoanalysis with a minimal blood sample volume (3 μL) and a total assay time 3 times shorter than that of the conventional enzyme-linked immunosorbent assay (ELISA). |
---|---|
AbstractList | Localized surface plasmon resonance (LSPR) nanoplasmonic effects allow for label-free, real-time detection of biomolecule binding events on a nanostructured metallic surface with simple optics and sensing tunability. Despite numerous reports on LSPR bionanosensing in the past, no study thus far has applied the technique for a cytokine secretion assay using clinically relevant immune cells from human blood. Cytokine secretion assays, a technique to quantify intercellular-signaling proteins secreted by blood immune cells, allow determination of the functional response of the donor's immune cells, thus providing valuable information about the immune status of the donor. However, implementation of LSPR bionanosensing in cellular functional immunoanalysis based on a cytokine secretion assay poses major challenges primarily owing to its limited sensitivity and a lack of sufficient sample handling capability. In this paper, we have developed a label-free LSPR biosensing technique to detect cell-secreted tumor necrosis factor (TNF)-α cytokines in clinical blood samples. Our approach integrates LSPR bionanosensors in an optofluidic platform that permits trapping and stimulation of target immune cells in a microfluidic chamber with optical access for subsequent cytokine detection. The on-chip spatial confinement of the cells is the key to rapidly increasing a cytokine concentration high enough for detection by the LSPR setup, thereby allowing the assay time and sample volume to be significantly reduced. We have successfully applied this approach first to THP-1 cells and then later to CD45 cells isolated directly from human blood. Our LSPR optofluidics device allows for detection of TNF-α secreted from cells as few as 1000, which translates into a nearly 100 times decrease in sample volume than conventional cytokine secretion assay techniques require. We achieved cellular functional immunoanalysis with a minimal blood sample volume (3 μL) and a total assay time 3 times shorter than that of the conventional enzyme-linked immunosorbent assay (ELISA). |
Author | Seo, Jung Hwan Chen, Pengyu Fu, Jianping Oh, Bo-Ram Huang, Nien-Tsu Chen, Weiqiang Kurabayashi, Katsuo Shanley, Thomas P Cornell, Timothy T |
AuthorAffiliation | Hongik University University of Michigan National Taiwan University |
AuthorAffiliation_xml | – name: University of Michigan – name: National Taiwan University – name: Hongik University |
Author_xml | – sequence: 1 givenname: Bo-Ram surname: Oh fullname: Oh, Bo-Ram – sequence: 2 givenname: Nien-Tsu surname: Huang fullname: Huang, Nien-Tsu – sequence: 3 givenname: Weiqiang surname: Chen fullname: Chen, Weiqiang – sequence: 4 givenname: Jung Hwan surname: Seo fullname: Seo, Jung Hwan – sequence: 5 givenname: Pengyu surname: Chen fullname: Chen, Pengyu – sequence: 6 givenname: Timothy T surname: Cornell fullname: Cornell, Timothy T – sequence: 7 givenname: Thomas P surname: Shanley fullname: Shanley, Thomas P – sequence: 8 givenname: Jianping surname: Fu fullname: Fu, Jianping – sequence: 9 givenname: Katsuo surname: Kurabayashi fullname: Kurabayashi, Katsuo email: katsuo@umich.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24568576$$D View this record in MEDLINE/PubMed |
BookMark | eNptkV1LwzAUhoNM3Ide-AekN4JeVJO2SdMbQYdzA9ELHehVOGvTmdEmM2mE_Xurm0XBq_PCeXjPxztEPW20ROiY4AuCI3KpdYJZnGK_hwYki1mIOXvpdZqSPho6t8KYpjxlB6gfJZRxmrIBep3pRi4tNLIIHkCbdQWuNlrlwZPUTullUBobjGVV-QpsMPE6b5TRUAWzuvbaQCs3Trlg_g1PfQ06uKmMKQ7RfgmVk0e7OkLzye3zeBreP97Nxtf3ISQpaUKIMCMszzgHoAmLoEx5wSmPaZTFC84XMStIWSQpzQEYlhmQeFGUQEgRA0AUj9DV1nftF7UscqkbC5VYW1WD3QgDSvztaPUmluZDJBgnUUZag7OdgTXvXrpG1Mrl7cWgpfFOEEpwgknEcIueb9HcGuesLLsxBIuvKEQXRcue_N6rI39-3wKnWwByJ1bG2_aV7h-jT4b_lDY |
CitedBy_id | crossref_primary_10_1002_smtd_201700197 crossref_primary_10_1007_s00604_017_2419_5 crossref_primary_10_1364_OME_5_000818 crossref_primary_10_1515_nanoph_2016_0156 crossref_primary_10_1039_C8LC00605A crossref_primary_10_5757_ASCT_2022_31_2_35 crossref_primary_10_1016_j_aca_2014_10_009 crossref_primary_10_1016_j_addr_2015_09_005 crossref_primary_10_1016_j_snb_2021_129725 crossref_primary_10_7567_JJAP_57_03EC03 crossref_primary_10_1002_smll_201702497 crossref_primary_10_1021_nn502549v crossref_primary_10_1021_acssensors_9b01786 crossref_primary_10_1002_admi_202101291 crossref_primary_10_1002_wsbm_1510 crossref_primary_10_1016_j_bios_2023_115285 crossref_primary_10_1016_j_tibtech_2014_09_003 crossref_primary_10_1186_s12951_021_01188_6 crossref_primary_10_1515_nanoph_2016_0101 crossref_primary_10_1039_C5LC01046B crossref_primary_10_1016_j_bios_2015_07_022 crossref_primary_10_1002_adhm_201801478 crossref_primary_10_1039_D0LC01173H crossref_primary_10_1021_acs_analchem_6b02353 crossref_primary_10_1038_s41598_020_62894_z crossref_primary_10_1116_1_4967748 crossref_primary_10_1088_2516_1091_ab7cc4 crossref_primary_10_1007_s13206_016_0409_z crossref_primary_10_1016_j_bios_2015_07_068 crossref_primary_10_1016_j_talanta_2020_120758 crossref_primary_10_1109_MNANO_2023_3297104 crossref_primary_10_3390_nano7050100 crossref_primary_10_1007_s00216_021_03203_z crossref_primary_10_1016_j_jim_2024_113648 crossref_primary_10_1146_annurev_anchem_061318_115055 crossref_primary_10_1016_j_bpj_2016_03_018 crossref_primary_10_1007_s12257_022_0088_7 crossref_primary_10_1021_acssensors_6b00619 crossref_primary_10_1002_smll_201800698 crossref_primary_10_1016_j_bios_2021_113156 crossref_primary_10_1016_j_bios_2021_113150 crossref_primary_10_1021_acsnano_2c09018 crossref_primary_10_1021_acsnano_5b00396 crossref_primary_10_1109_JPROC_2016_2624340 crossref_primary_10_1080_10667857_2018_1527803 crossref_primary_10_1109_MNANO_2020_2966205 crossref_primary_10_3390_s20030944 crossref_primary_10_1021_acssensors_0c01180 crossref_primary_10_1016_j_tibtech_2015_09_012 crossref_primary_10_3390_mi11010107 crossref_primary_10_1016_j_bios_2016_01_020 crossref_primary_10_3390_bios11120500 crossref_primary_10_1021_acschembio_7b01029 crossref_primary_10_1016_j_aca_2021_339307 crossref_primary_10_1063_5_0098128 crossref_primary_10_1007_s11468_015_9969_3 crossref_primary_10_1021_acsami_2c14748 crossref_primary_10_1088_0957_4484_27_29_295101 crossref_primary_10_1117_1_JNP_14_036004 crossref_primary_10_1002_smll_202101743 crossref_primary_10_1021_acs_analchem_5b00287 crossref_primary_10_1186_s12879_023_08291_z crossref_primary_10_1021_nn503826r crossref_primary_10_1021_acssensors_6b00240 crossref_primary_10_15406_jnmr_2016_04_00094 crossref_primary_10_1016_j_bios_2019_111528 crossref_primary_10_3390_chemosensors10010015 crossref_primary_10_1063_1_5079815 crossref_primary_10_1021_acsnano_7b01162 crossref_primary_10_1021_acssensors_0c01774 crossref_primary_10_1039_C7LC00277G crossref_primary_10_3389_fsens_2022_789771 crossref_primary_10_3390_mi11010052 crossref_primary_10_1016_j_sna_2022_113401 crossref_primary_10_3390_bios12111021 crossref_primary_10_1007_s40820_021_00598_3 |
Cites_doi | 10.1016/0955-0674(91)90135-L 10.1038/ncomms3381 10.1001/jama.2011.1829 10.1146/annurev.physchem.58.032806.104607 10.1049/ip-com:20040966 10.1021/cr100313v 10.1021/jp106912p 10.3390/s110807644 10.1016/j.aca.2008.03.022 10.1039/c2lc40619e 10.1016/j.jtcvs.2011.09.011 10.1143/JJAP.47.1337 10.1002/adhm.201200378 10.1097/01.shk.0000145206.28812.60 10.1586/eri.12.102 10.3390/s100807323 10.1039/c3lc41388h 10.1016/j.bios.2011.10.047 10.1021/ac202363t 10.1016/j.stam.2005.03.019 10.1021/nn7003734 |
ContentType | Journal Article |
Copyright | Copyright © 2014 American Chemical Society Copyright © 2014 American Chemical Society 2014 American Chemical Society |
Copyright_xml | – notice: Copyright © 2014 American Chemical Society – notice: Copyright © 2014 American Chemical Society 2014 American Chemical Society |
DBID | N~. CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1021/nn406370u |
DatabaseName | American Chemical Society (ACS) Open Access Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: N~. name: American Chemical Society (ACS) Open Access url: https://pubs.acs.org sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 1936-086X |
EndPage | 2676 |
ExternalDocumentID | 10_1021_nn406370u 24568576 h46031796 |
Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NICHD NIH HHS grantid: K08HD062142 – fundername: NHLBI NIH HHS grantid: R01 HL119542 – fundername: NICHD NIH HHS grantid: K08 HD062142 |
GroupedDBID | - 23M 4.4 53G 55A 5GY 7~N AABXI ABMVS ABUCX ACGFS ACS AEESW AENEX AFEFF ALMA_UNASSIGNED_HOLDINGS AQSVZ CS3 EBS ED ED~ EJD F5P GNL IH9 IHE JG JG~ LG6 N~. P2P RNS ROL UI2 VF5 VG9 W1F XKZ YZZ --- .K2 5VS 6J9 AAHBH ABJNI ABQRX ACBEA ACGFO ADHLV AHGAQ BAANH CGR CUPRZ CUY CVF ECM EIF GGK NPM AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-a471t-a20616c988aa5462af78d85835293b88b36d1fd475caa60e9a13bdfa11d3aaa23 |
IEDL.DBID | N~. |
ISSN | 1936-0851 |
IngestDate | Tue Sep 17 21:16:41 EDT 2024 Fri Aug 16 11:57:17 EDT 2024 Fri Aug 23 00:24:50 EDT 2024 Sat Sep 28 08:02:27 EDT 2024 Thu Aug 27 13:45:07 EDT 2020 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | nanoplasmonic sensing cellular immunoanalysis tumor necrosis factor alpha (TNF-α) optofluidics localized surface plasmon resonance (LSPR) |
Language | English |
License | http://pubs.acs.org/page/policy/authorchoice_termsofuse.html |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-a471t-a20616c988aa5462af78d85835293b88b36d1fd475caa60e9a13bdfa11d3aaa23 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | http://dx.doi.org/10.1021/nn406370u |
PMID | 24568576 |
PQID | 1510401260 |
PQPubID | 23479 |
PageCount | 10 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_4004291 proquest_miscellaneous_1510401260 crossref_primary_10_1021_nn406370u pubmed_primary_24568576 acs_journals_10_1021_nn406370u |
ProviderPackageCode | JG~ 55A AABXI GNL VF5 XKZ 7~N VG9 W1F ACS AEESW AFEFF ABMVS ABUCX IH9 AQSVZ ED~ N~. UI2 |
PublicationCentury | 2000 |
PublicationDate | 2014-03-25 |
PublicationDateYYYYMMDD | 2014-03-25 |
PublicationDate_xml | – month: 03 year: 2014 text: 2014-03-25 day: 25 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | ACS nano |
PublicationTitleAlternate | ACS Nano |
PublicationYear | 2014 |
Publisher | American Chemical Society |
Publisher_xml | – name: American Chemical Society |
References | 22148421 - Anal Chem. 2012 Jan 3;84(1):232-40 1652987 - Curr Opin Cell Biol. 1991 Apr;3(2):171-5 23979039 - Nat Commun. 2013;4:2381 1805528 - Acta Med Port. 1991 Dec;4 Suppl 1:20S-27S 21648956 - Chem Rev. 2011 Jun 8;111(6):3828-57 21660207 - J Phys Chem C Nanomater Interfaces. 2011 Feb 10;115(5):1410-1414 23199394 - Expert Rev Anti Infect Ther. 2012 Oct;10(10):1079-81 22099957 - Biosens Bioelectron. 2012 Jan 15;31(1):567-70 18420049 - Anal Chim Acta. 2008 May 5;614(2):182-9 22164036 - Sensors (Basel). 2011;11(8):7644-55 22163605 - Sensors (Basel). 2010;10(8):7323-46 22892681 - Lab Chip. 2012 Oct 21;12(20):4093-101 21996297 - J Thorac Cardiovasc Surg. 2012 May;143(5):1160-1166.e1 17067281 - Annu Rev Phys Chem. 2007;58:267-97 15614136 - Shock. 2005 Jan;23(1):80-7 23592024 - Lab Chip. 2013 Jun 7;13(11):2066-74 2031186 - Science. 1991 May 24;252(5009):1164-7 22187279 - JAMA. 2011 Dec 21;306(23):2594-605 23626950 - Front Oncol. 2013 Apr 22;3:98 16441155 - IEE Proc Nanobiotechnol. 2005 Feb;152(1):13-32 19206599 - ACS Nano. 2008 Apr;2(4):687-92 19053435 - J Am Chem Soc. 2008 Dec 17;130(50):17095-105 23335389 - Adv Healthc Mater. 2013 Jul;2(7):965-75 Huang T. (ref3/cit3) 2008; 130 Mayer K. M. (ref15/cit15) 2011; 111 Benton H. P. (ref6/cit6) 1991; 3 Prime K. L. (ref20/cit20) 1991; 252 Bellapadrona G. (ref2/cit2) 2012; 84 Stuart D. A. (ref4/cit4) 2005; 152 Shen Y. (ref10/cit10) 2013; 4 Endo T. (ref12/cit12) 2008; 614 Salehi-Reyhani A. (ref18/cit18) 2013; 13 Zhou Q. (ref9/cit9) 2012; 10 Boomer J. S. (ref7/cit7) 2011; 306 Endo T. (ref11/cit11) 2005; 6 Guo L. H. (ref17/cit17) 2012; 31 Huang N. T. (ref24/cit24) 2012; 12 Hall W. P. (ref13/cit13) 2011; 115 Chen W. (ref23/cit23) 2013; 2 Cornell T. T. (ref25/cit25) 2012; 143 Hiep H. M. (ref14/cit14) 2008; 47 ref21/cit21 Martins E Silva J. (ref5/cit5) 1991; 4 Mayer K. M. (ref1/cit1) 2008; 2 Mitchell J. (ref16/cit16) 2010; 10 Lazar J. (ref22/cit22) 2011; 11 Chen W. (ref8/cit8) 2013; 3 Nimah M. (ref26/cit26) 2005; 23 Willets K. A. (ref19/cit19) 2007; 58 |
References_xml | – ident: ref21/cit21 – volume: 130 start-page: 17095 year: 2008 ident: ref3/cit3 publication-title: J. Am. Med. Assoc. contributor: fullname: Huang T. – volume: 3 start-page: 171 year: 1991 ident: ref6/cit6 publication-title: Curr. Opin. iCell Biol. doi: 10.1016/0955-0674(91)90135-L contributor: fullname: Benton H. P. – volume: 4 start-page: 2381 year: 2013 ident: ref10/cit10 publication-title: Nat. Commun. doi: 10.1038/ncomms3381 contributor: fullname: Shen Y. – volume: 306 start-page: 2594 year: 2011 ident: ref7/cit7 publication-title: J. Am. Med. Assoc. doi: 10.1001/jama.2011.1829 contributor: fullname: Boomer J. S. – volume: 58 start-page: 267 year: 2007 ident: ref19/cit19 publication-title: Annu. Rev. Phys. Chem. doi: 10.1146/annurev.physchem.58.032806.104607 contributor: fullname: Willets K. A. – volume: 152 start-page: 13 year: 2005 ident: ref4/cit4 publication-title: IEE Proc.: Nanobiotechnol. doi: 10.1049/ip-com:20040966 contributor: fullname: Stuart D. A. – volume: 111 start-page: 3828 year: 2011 ident: ref15/cit15 publication-title: Chem. Rev. doi: 10.1021/cr100313v contributor: fullname: Mayer K. M. – volume: 4 start-page: 20 year: 1991 ident: ref5/cit5 publication-title: Acta Med. Port. contributor: fullname: Martins E Silva J. – volume: 115 start-page: 1410 year: 2011 ident: ref13/cit13 publication-title: J. Phys. Chem. C doi: 10.1021/jp106912p contributor: fullname: Hall W. P. – volume: 11 start-page: 7644 year: 2011 ident: ref22/cit22 publication-title: Sensors doi: 10.3390/s110807644 contributor: fullname: Lazar J. – volume: 614 start-page: 182 year: 2008 ident: ref12/cit12 publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2008.03.022 contributor: fullname: Endo T. – volume: 252 start-page: 1164 year: 1991 ident: ref20/cit20 publication-title: Sci. Technol. Adv. Mater. contributor: fullname: Prime K. L. – volume: 12 start-page: 4093 year: 2012 ident: ref24/cit24 publication-title: Lab Chip doi: 10.1039/c2lc40619e contributor: fullname: Huang N. T. – volume: 3 start-page: 1 year: 2013 ident: ref8/cit8 publication-title: Front. Oncol. contributor: fullname: Chen W. – volume: 143 start-page: 1160 year: 2012 ident: ref25/cit25 publication-title: J. Thorac. Cardiovasc. Surg. doi: 10.1016/j.jtcvs.2011.09.011 contributor: fullname: Cornell T. T. – volume: 47 start-page: 1337 year: 2008 ident: ref14/cit14 publication-title: Jpn. J. Appl. Phys. doi: 10.1143/JJAP.47.1337 contributor: fullname: Hiep H. M. – volume: 2 start-page: 965 year: 2013 ident: ref23/cit23 publication-title: Adv. Healthcare Mater. doi: 10.1002/adhm.201200378 contributor: fullname: Chen W. – volume: 23 start-page: 80 year: 2005 ident: ref26/cit26 publication-title: Shock doi: 10.1097/01.shk.0000145206.28812.60 contributor: fullname: Nimah M. – volume: 10 start-page: 1079 year: 2012 ident: ref9/cit9 publication-title: Expert Rev. Anti-Infect. Ther. doi: 10.1586/eri.12.102 contributor: fullname: Zhou Q. – volume: 10 start-page: 7323 year: 2010 ident: ref16/cit16 publication-title: Sensors doi: 10.3390/s100807323 contributor: fullname: Mitchell J. – volume: 13 start-page: 2066 year: 2013 ident: ref18/cit18 publication-title: Lab Chip doi: 10.1039/c3lc41388h contributor: fullname: Salehi-Reyhani A. – volume: 31 start-page: 567 year: 2012 ident: ref17/cit17 publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2011.10.047 contributor: fullname: Guo L. H. – volume: 84 start-page: 232 year: 2012 ident: ref2/cit2 publication-title: Anal. Chem. doi: 10.1021/ac202363t contributor: fullname: Bellapadrona G. – volume: 6 start-page: 491 year: 2005 ident: ref11/cit11 publication-title: Sci. Technol. Adv. Mater. doi: 10.1016/j.stam.2005.03.019 contributor: fullname: Endo T. – volume: 2 start-page: 687 year: 2008 ident: ref1/cit1 publication-title: ACS Nano doi: 10.1021/nn7003734 contributor: fullname: Mayer K. M. |
SSID | ssj0057876 |
Score | 2.4785545 |
Snippet | Localized surface plasmon resonance (LSPR) nanoplasmonic effects allow for label-free, real-time detection of biomolecule binding events on a nanostructured... |
SourceID | pubmedcentral proquest crossref pubmed acs |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 2667 |
SubjectTerms | Calibration Cytokines - blood Equipment Design Humans Immunoassay - instrumentation Immunoassay - methods Microfluidic Analytical Techniques Nanotechnology - instrumentation Nanotechnology - methods Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods |
Title | Integrated Nanoplasmonic Sensing for Cellular Functional Immunoanalysis Using Human Blood |
URI | http://dx.doi.org/10.1021/nn406370u https://www.ncbi.nlm.nih.gov/pubmed/24568576 https://search.proquest.com/docview/1510401260 https://pubmed.ncbi.nlm.nih.gov/PMC4004291 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3JTsMwEB2VcoEDYqcslVmugdhOYucIFVWLRC-lUjlFduIAUkkq2l75dsZJU7Us4hzHimbGfm884xeAq8RwqrRIHU9r7nhISh2pXeMwoyRCrGYqtecdj72gM_Aehv6wBpd_VPAZvckyxBwu3NkarDNBi9XX-7yutlsbcUFZOsbUGPlDJR-0_KqFnniyCj0_-OT3tsglnGlvw9acIJLb0qM7UDPZLmwuyQbuwXO30nhICO6O-RgZ8LuVuCV9246evRBkoqRlRiPbY0raCF3liR_p2tsguZoLkZCiX4AU5_jkznaw78Ogff_U6jjzXyQ4ClFl6iiGeBzEoZRK-V5gLSsT6VtaFXItpeZBQtPEE36sVOCaUFGuk1RRmnClFOMHUM_yzBwBiXUgNK5nwUXqhcagxxg1khsqktDlugFNtGE0D_FJVFSvGY0WRm7ARWXeaFxKZfw26LwyfISBbKsTKjP5DOfD3QGTPcyvGnBYOmIxja3OSsyMGiBWXLQYYEWyV59kb6-FWLZXQC49_u_rT2ADuZBn28uYfwr16cfMnCHfmOom8u1Wv1lE3Re8S9PD |
link.rule.ids | 230,315,786,790,891,2782,27107,27111,27955,27956,57091,57115,57141,57165 |
linkProvider | American Chemical Society |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV07T8MwED5BGYAB8SY8ikGsgTpOYmeEiqqFtgutVKbIbhxAKklF25XfzjmP0gIScxwrurv4-853_gxwFWlGpeKx7SrFbBdJqS1UTduOlgIhVjkyNvsdna7f7LsPA29QyOSYszD4EROcaZIV8b_VBehNkiD0MF6brcKax5HHmEsuP6_LVdcEnp9XkDFDRhpRqggtvmoQaDhZRqBftPJnd-QC3DS2YavgieQ2d-wOrOhkFzYX1AP34LlVSj1EBBfJdIxE-N0o3ZIn05WevBAkpKSuRyPTakoaiGD5xh9pmUMhqSz0SEjWNkCy7XxyZxrZ96HfuO_Vm3ZxU4ItEVymtnQQlv1hIISUnusbA4tIeIZdBUwJoZgf0ThyuTeU0q_pQFKmolhSGjEppcMOoJKkiT4CMlQ-V_hbc8ZjN9AaHedQLZimPApqTFlQRRuGRaRPwqyI7dBwbmQLLkvzhuNcMeOvQRel4UOMZ1OkkIlOZzgfLhKY82GaZcFh7oj5NKZIKzBBsoAvuWg-wGhlLz9J3l4zzWw3Q156_N_Xn8N6s9dph-1W9_EENpAeuabjzPFOoTL9mOkzpCBTVc1i7wuYN9h6 |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3JTsMwEB1BkRAcEDtlKQZxDdRxFucIhaplqZAACU6RXTuAVNKKtle-nZksVQtInONY0czY741n_AJwYqzgSoeJ42ktHA9JqSN13TquVRIhVrsqofOOu07QevKun_3nIlGkuzD4EUOcaZgV8WlVD0xSKAzwszRF-BFhfTwPCz4SfWrh63ydljsvBV-QV5ExS0YqUSoJTb9KKNQdzqLQL2r5s0NyCnKaq7BScEV2njt3DeZsug7LUwqCG_DSLuUeDMONsj9AMvxBarfsgTrT01eGpJQ1bK9H7aasiSiWH_6xNl0M6atCk4RlrQMsO9JnF9TMvglPzavHRssp_pbgKASYkaNchOagG0mplO8FZGRppE8MKxJaSi0CwxPjhX5XqaBuI8WFNoni3AillCu2oJL2U7sDrKuDUOPSDkWYeJG16DyXWyksD01UF7oKNbRhXET7MM4K2S6PJ0auwnFp3niQq2b8NeioNHyMMU2FCpXa_hjnw40C8z5MtaqwnTtiMg0VaiUmSVUIZ1w0GUB62bNP0ve3TDfby9CX7_739YeweH_ZjG_bnZs9WEKG5FHTmevvQ2X0ObYHyEJGupaF3jfYrtmQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Integrated+nanoplasmonic+sensing+for+cellular+functional+immunoanalysis+using+human+blood&rft.jtitle=ACS+nano&rft.au=Oh%2C+Bo-Ram&rft.au=Huang%2C+Nien-Tsu&rft.au=Chen%2C+Weiqiang&rft.au=Seo%2C+Jung+Hwan&rft.date=2014-03-25&rft.eissn=1936-086X&rft.volume=8&rft.issue=3&rft.spage=2667&rft_id=info:doi/10.1021%2Fnn406370u&rft_id=info%3Apmid%2F24568576&rft.externalDocID=24568576 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1936-0851&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1936-0851&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1936-0851&client=summon |