Purification and Characterization of a Trypsin Inhibitor from Plathymenia foliolosa Seeds

• Published in: Journal of agricultural and food chemistry Vol. 56; no. 23; pp. 11348 - 11355
• Main Authors: da Silveira Ramos, Vanessa, de Souza Silva, Gilvânia, das Graças Machado Freire, Maria, Lima Tavares Machado, Olga, Postali Parra, José Roberto, Rodrigues Macedo, Maria Lígia
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 10.12.2008
• Subjects: Amino Acid Sequence; Anticarsia gemmatalis; Biological and medical sciences; chymotrypsin; Crop and Animal Protection Chemistry; Diatraea saccharalis; enzyme inhibition; Fabaceae - chemistry; Fabaceae - genetics; Fabaceae - metabolism; Food industries; Fundamental and applied biological sciences. Psychology; gel electrophoresis; insect pests; isolation; Kinetics; Kunitz family; larvae; midgut; Molecular Sequence Data; N-terminal sequence; pest protease; plant proteins; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Plathymenia; Plathymenia foliolosa; seeds; Seeds - chemistry; Seeds - genetics; Seeds - metabolism; Sequence Alignment; trypsin; trypsin inhibitor; trypsin inhibitors; Trypsin Inhibitors - chemistry; Trypsin Inhibitors - genetics; Trypsin Inhibitors - isolation & purification; Trypsin Inhibitors - metabolism; Kunitz family; Plathymenia foliolosa; trypsin inhibitor; N-terminal sequence; pest protease; Seeds; Purification; Serine endopeptidases; Enzyme; Characterization; Peptidases; Trypsin; Pest; N terminal-Sequence; Hydrolases; Inhibitor

A novel trypsin inhibitor (PFTI) was isolated from Plathymenia foliolosa (Benth.) seeds by gel filtration chromatography on a Sephadex G-100, DEAE-Sepharose, and trypsin-Sepharose columns. By SDS-PAGE, PFTI yielded a single band with a M r of 19 kDa. PFTI inhibited bovine trypsin and bovine chymotry...