Analysis of Tamoxifen and Its Metabolites by On-Line Capillary Electrophoresis−Electrospray Ionization Mass Spectrometry Employing Nonaqueous Media Containing Surfactants

• Published in: Analytical chemistry (Washington) Vol. 68; no. 4; pp. 668 - 674
• Main Authors: Lu, Wenzhe, Poon, Grace K, Carmichael, Paul L, Cole, Richard B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.02.1996
• Subjects: Analysis; Animals; Antineoplastic Agents, Hormonal - analysis; Antineoplastic Agents, Hormonal - pharmacokinetics; Biological and medical sciences; Biotransformation; Cells, Cultured; Chemistry; Drugs; Electrophoresis, Capillary; General pharmacology; Mass Spectrometry; Medical sciences; Mice; Microsomes, Liver - chemistry; Pharmacology. Drug treatments; Rats; Scientific imaging; Surface-Active Agents; Tamoxifen - analysis; Tamoxifen - pharmacokinetics; Antineoplastic agent; Cell culture; Metabolite; Rodentia; In vitro; Electrospray; Chemical ionization; Tamoxifene; Capillary tube; Vertebrata; Mammalia; Hepatocyte; Investigation method; Mouse; Animal; Electrophoresis; Mass spectrometry

On-line capillary electrophoresis−electrospray ionization mass spectrometry (CE−ESMS) has been employed for the analysis of metabolites of the anticancer drug tamoxifen. Nonaqueous (methanol) CE electrolyte provided better resolution and detection sensitivity compared to aqueous systems or highly aq...