Fluorophore Targeting to Cellular Proteins via Enzyme-Mediated Azide Ligation and Strain-Promoted Cycloaddition

• Published in: Journal of the American Chemical Society Vol. 134; no. 8; pp. 3720 - 3728
• Main Authors: Yao, Jennifer Z, Uttamapinant, Chayasith, Poloukhtine, Andrei, Baskin, Jeremy M, Codelli, Julian A, Sletten, Ellen M, Bertozzi, Carolyn R, Popik, Vladimir V, Ting, Alice Y
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 29.02.2012
• Subjects: Animals; Azides - chemistry; Azides - metabolism; Biocatalysis; Cells, Cultured; Chlorocebus aethiops; COS Cells; Cyclization; Cyclooctanes - chemistry; Cyclooctanes - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; HEK293 Cells; HeLa Cells; Humans; Ligases - chemistry; Ligases - genetics; Ligases - metabolism; Luminescent Proteins - chemistry; Luminescent Proteins - metabolism; Molecular Structure; Mutation

Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Previously, we reported targeting of the blue fluorophore coumarin to cellular proteins fused to a 13-amino acid recognition s...