Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

• Published in: mSystems Vol. 9; no. 5; p. e0025024
• Main Authors: Pšeničnik, Alen, Slemc, Lucija, Avbelj, Martina, Tome, Miha, Šala, Martin, Herron, Paul, Shmatkov, Maksym, Petek, Marko, Baebler, Špela, Mrak, Peter, Hranueli, Daslav, Starčević, Antonio, Hunter, Iain S., Petković, Hrvoje
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 16.05.2024
• Subjects: Amino acids; Anti-Bacterial Agents - biosynthesis; Antibiotics; Antimicrobial resistance; Applied and Industrial Microbiology; Biosynthesis; Chromosomes; Cryptic gene; Drug development; Gene clusters; Gene deletion; Gene rearrangement; Genes; Genome, Bacterial; Genomes; Genomic analysis; Industrial strains; Metabolism; Metabolites; Microorganisms; Multigene Family - genetics; Mutagenesis; Natural products; Oxytetracycline; Oxytetracycline - biosynthesis; Research Article; Secondary metabolites; Streptomyces - drug effects; Streptomyces - genetics; Streptomyces - metabolism; Streptomyces rimosus; Streptomyces rimosus - genetics; Streptomyces rimosus - metabolism; Transcriptomes; genome reduction; oxytetracycline; antibiotic biosynthesis; cryptic metabolites

There is a critical need to develop novel antibiotics to combat antimicrobial resistance. Streptomyces species are very rich source of antibiotics, typically encoding 20–60 biosynthetic gene clusters (BGCs). However, under laboratory conditions, most are either silent or poorly expressed so that the...