Characterization of the Glutathione S -Transferases Involved in Styrene Degradation in Gordonia rubripertincta CWB2

• Published in: Microbiology spectrum Vol. 9; no. 1; p. e0047421
• Main Authors: Lienkamp, Anna C, Burnik, Jan, Heine, Thomas, Hofmann, Eckhard, Tischler, Dirk
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 03.09.2021
• Subjects: Actinobacteria; Actinobacteria - classification; Actinobacteria - enzymology; Actinobacteria - genetics; Actinobacteria - metabolism; Biotransformation; Environmental Microbiology; Epoxy Compounds; Escherichia coli - genetics; glutathione; Glutathione - metabolism; glutathione S-transferases; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Ibuprofen; Phylogeny; Plasmids; Research Article; styrene metabolism; styrene oxide; Styrenes - metabolism; biotransformation; styrene metabolism; microbial ibuprofen production; glutathione; Actinobacteria; styrene oxide; glutathione S-transferases

The glutathione -transferases carried on the plasmid for the styrene-specific degradation pathway in the Gordonia rubripertincta CWB2 were heterologously expressed in Escherichia coli. Both enzymes were purified via affinity chromatography and subjected to activity investigations. StyI and StyJ disp...