Simulation and Experimental Assembly of DNA–Graft Copolymer Micelles with Controlled Morphology
Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over alternative delivery strategies. Notably, recent research has shown that the shape of these particles can be tuned, which makes it possible to gain un...
Saved in:
Published in | ACS biomaterials science & engineering Vol. 1; no. 6; pp. 448 - 455 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
08.06.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over alternative delivery strategies. Notably, recent research has shown that the shape of these particles can be tuned, which makes it possible to gain understanding of their shape-dependent transfection properties. Whereas earlier methods achieved shape tuning through the use of block copolymers and variation of solvent polarity, here we demonstrate through a combined experimental and computational approach that the same degree of shape control can be achieved through the use of graft copolymers that are easier to synthesize and provide a wider range of parameters for shape control. Moreover, the approach presented here does not require the use of organic solvents. The simulation work provides insight into the mechanism governing the shape variation as well as an effective model to guide further design of nonviral gene-delivery vectors. Our experimental findings offer important opportunities for the facile and large-scale synthesis of biocompatible gene-delivery vectors with well-controlled shape and tunable transfection properties. The in vitro study shows that both micelle shape and transfection efficiency are strongly correlated with the key structural parameters of the graft copolymer carriers. |
---|---|
AbstractList | Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over alternative delivery strategies. Notably, recent research has shown that the shape of these particles can be tuned, which makes it possible to gain understanding of their shape-dependent transfection properties. Whereas earlier methods achieved shape tuning through the use of block copolymers and variation of solvent polarity, here we demonstrate through a combined experimental and computational approach that the same degree of shape control can be achieved through the use of graft copolymers that are easier to synthesize and provide a wider range of parameters for shape control. Moreover, the approach presented here does not require the use of organic solvents. The simulation work provides insight into the mechanism governing the shape variation as well as an effective model to guide further design of non-viral gene-delivery vectors. Our experimental findings offer important opportunities for the facile and large-scale synthesis of biocompatible gene-delivery vectors with well-controlled shape and tunable transfection properties. The
study shows that both micelle shape and transfection efficiency are strongly correlated with the key structural parameters of the graft copolymer carriers. Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over alternative delivery strategies. Notably, recent research has shown that the shape of these particles can be tuned, which makes it possible to gain understanding of their shape-dependent transfection properties. Whereas earlier methods achieved shape tuning through the use of block copolymers and variation of solvent polarity, here we demonstrate through a combined experimental and computational approach that the same degree of shape control can be achieved through the use of graft copolymers that are easier to synthesize and provide a wider range of parameters for shape control. Moreover, the approach presented here does not require the use of organic solvents. The simulation work provides insight into the mechanism governing the shape variation as well as an effective model to guide further design of non-viral gene-delivery vectors. Our experimental findings offer important opportunities for the facile and large-scale synthesis of biocompatible gene-delivery vectors with well-controlled shape and tunable transfection properties. The in vitro study shows that both micelle shape and transfection efficiency are strongly correlated with the key structural parameters of the graft copolymer carriers. Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over alternative delivery strategies. Notably, recent research has shown that the shape of these particles can be tuned, which makes it possible to gain understanding of their shape-dependent transfection properties. Whereas earlier methods achieved shape tuning through the use of block copolymers and variation of solvent polarity, here we demonstrate through a combined experimental and computational approach that the same degree of shape control can be achieved through the use of graft copolymers that are easier to synthesize and provide a wider range of parameters for shape control. Moreover, the approach presented here does not require the use of organic solvents. The simulation work provides insight into the mechanism governing the shape variation as well as an effective model to guide further design of nonviral gene-delivery vectors. Our experimental findings offer important opportunities for the facile and large-scale synthesis of biocompatible gene-delivery vectors with well-controlled shape and tunable transfection properties. The in vitro study shows that both micelle shape and transfection efficiency are strongly correlated with the key structural parameters of the graft copolymer carriers. |
Author | Mao, Hai-Quan Wei, Zonghui Williford, John-Michael Qu, Wei Ng, Shirley Luijten, Erik Ren, Yong Huang, Kevin |
AuthorAffiliation | Johns Hopkins University Graduate Program in Applied Physics Northwestern University Translational Tissue Engineering Center and Whitaker Biomedical Engineering Institute Department of Physics and Astronomy Department of Chemical and Biomolecular Engineering Department of Materials Science and Engineering Department of Materials Science and Engineering, Whiting School of Engineering Department of Biomedical Engineering Department of Engineering Sciences and Applied Mathematics Johns Hopkins School of Medicine |
AuthorAffiliation_xml | – name: – name: Department of Physics and Astronomy – name: Johns Hopkins School of Medicine – name: Department of Chemical and Biomolecular Engineering – name: Department of Materials Science and Engineering, Whiting School of Engineering – name: Northwestern University – name: Department of Engineering Sciences and Applied Mathematics – name: Department of Biomedical Engineering – name: Translational Tissue Engineering Center and Whitaker Biomedical Engineering Institute – name: Graduate Program in Applied Physics – name: Department of Materials Science and Engineering – name: Johns Hopkins University – name: Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, United States – name: Graduate Program in Applied Physics, Northwestern University, Evanston, Illinois 60208, United States – name: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States – name: Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois 60208, United States – name: δ Department of Physics and Astronomy, Northwestern University, Evanston, Illinois 60208, United States – name: ς Translational Tissue Engineering Center and Whitaker Biomedical Engineering Institute, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, United States – name: Department of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States – name: Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States |
Author_xml | – sequence: 1 givenname: Zonghui surname: Wei fullname: Wei, Zonghui – sequence: 2 givenname: Yong surname: Ren fullname: Ren, Yong – sequence: 3 givenname: John-Michael surname: Williford fullname: Williford, John-Michael – sequence: 4 givenname: Wei surname: Qu fullname: Qu, Wei – sequence: 5 givenname: Kevin surname: Huang fullname: Huang, Kevin – sequence: 6 givenname: Shirley surname: Ng fullname: Ng, Shirley – sequence: 7 givenname: Hai-Quan surname: Mao fullname: Mao, Hai-Quan email: hmao@jhu.edu – sequence: 8 givenname: Erik surname: Luijten fullname: Luijten, Erik email: luijten@northwestern.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29399627$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkcFu1DAQhi3UipbSVwAfuWyxYzuOL0irpRSkthzo3XKScdeVYwc7KeyNd-ANeRK82m1VuPRke_zPN_Prf4UOQgyA0FtKziip6HvT5dbFwUyQnPH5TLSEkIa8QMcVk2yhGtkcPLkfodOc74qEskZwzl-io0oxpepKHiPzzQ2zN5OLAZvQ4_OfY6EOECbj8TJnGFq_wdHij9fLP79-XyRjJ7yKY_SbARK-ch14Dxn_cNO61MOUYnn3-CqmcR19vN28Roe2bAmn-_ME3Xw6v1l9Xlx-vfiyWl4uDK_ptGhr2XQ9CBBSWGaJopRwyiUnwlrTcqiUrGVre6o60VVKEU6IkYwCVD1YdoI-7LDj3A7Qd8VBMl6PxYxJGx2N0__-BLfWt_FeC6nKaFIA7_aAFL_PkCc9uLx1ZwLEOWuqFGc1Z2IrlTtpl2LOCezjGEr0NiL9X0R6H1HpfPN0y8e-h0CKgO0EhaDv4pzCtv057F-Fk6jc |
CitedBy_id | crossref_primary_10_1021_acs_chemrev_0c00997 crossref_primary_10_1021_acsbiomaterials_5b00551 crossref_primary_10_1016_j_polymer_2016_12_068 crossref_primary_10_1063_1_4937384 crossref_primary_10_1021_acsabm_9b00171 crossref_primary_10_1088_1361_6528_aa6519 crossref_primary_10_1016_j_addr_2017_07_021 crossref_primary_10_1016_j_colsurfa_2016_04_033 crossref_primary_10_1016_j_nano_2019_04_004 |
Cites_doi | 10.1007/s11095-008-9697-x 10.1007/s11095-004-9003-5 10.1007/s11095-011-0436-3 10.1073/pnas.0801763105 10.1146/annurev-bioeng-071811-150124 10.1103/PhysRevLett.99.138302 10.1016/S0168-3659(02)00180-3 10.1126/science.237.4813.384 10.1021/ma034506o 10.1016/j.progpolymsci.2007.05.007 10.1002/anie.201104449 10.1002/adma.201202932 10.1016/j.addr.2009.04.016 10.1038/nrd1775 10.1038/nnano.2007.70 10.1021/bc025529v 10.1021/nl052396o 10.1073/pnas.1305000110 10.1016/0040-4039(95)00630-U 10.1021/bm1009574 10.1098/rsif.2008.0547.focus 10.1002/anie.200805895 10.1021/ma401093z 10.1073/pnas.0705898104 10.1517/17425247.2013.744964 10.1073/pnas.1216893110 10.1073/pnas.1308345110 10.1002/jgm.868 10.1038/mt.2012.79 10.1146/annurev.pc.10.100159.000535 10.1063/1.470698 10.1021/nn403069n |
ContentType | Journal Article |
Copyright | Copyright © American Chemical Society |
Copyright_xml | – notice: Copyright © American Chemical Society |
DBID | NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1021/acsbiomaterials.5b00080 |
DatabaseName | PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 2373-9878 |
EndPage | 455 |
ExternalDocumentID | 10_1021_acsbiomaterials_5b00080 29399627 d118939768 |
Genre | Journal Article |
GroupedDBID | 4.4 ABMVS ABUCX ACGFS ACS AEESW AFEFF ALMA_UNASSIGNED_HOLDINGS AQSVZ EBS EJD LG6 UI2 VF5 VG9 W1F 53G ABFRP ABQRX ADHLV AHGAQ BAANH CUPRZ GGK NPM AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-a461t-b678cde5e575f3f091104147405ffab4e29767bfd19c5c2990400a731ee2def3 |
IEDL.DBID | ACS |
ISSN | 2373-9878 |
IngestDate | Tue Sep 17 21:18:17 EDT 2024 Fri Aug 16 22:23:37 EDT 2024 Fri Aug 23 00:34:40 EDT 2024 Sun Jun 23 00:32:56 EDT 2024 Thu Aug 27 13:41:56 EDT 2020 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | true |
Issue | 6 |
Keywords | self-assembly shape control micelles DNA packaging transfection |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-a461t-b678cde5e575f3f091104147405ffab4e29767bfd19c5c2990400a731ee2def3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally. |
OpenAccessLink | http://csml.northwestern.edu/resources/Reprints/acsbiomat1.pdf |
PMID | 29399627 |
PQID | 1994364350 |
PQPubID | 23479 |
PageCount | 8 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_5796780 proquest_miscellaneous_1994364350 crossref_primary_10_1021_acsbiomaterials_5b00080 pubmed_primary_29399627 acs_journals_10_1021_acsbiomaterials_5b00080 |
ProviderPackageCode | ACS AEESW AFEFF VF5 VG9 ABMVS ABUCX AQSVZ W1F UI2 |
PublicationCentury | 2000 |
PublicationDate | 2015-Jun-08 |
PublicationDateYYYYMMDD | 2015-06-08 |
PublicationDate_xml | – month: 06 year: 2015 text: 2015-Jun-08 day: 08 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | ACS biomaterials science & engineering |
PublicationTitleAlternate | ACS Biomater. Sci. Eng |
PublicationYear | 2015 |
Publisher | American Chemical Society |
Publisher_xml | – name: American Chemical Society |
References | ref9/cit9 ref6/cit6 ref3/cit3 ref27/cit27 ref18/cit18 ref11/cit11 ref25/cit25 ref16/cit16 ref29/cit29 ref32/cit32 ref23/cit23 ref14/cit14 ref8/cit8 ref5/cit5 ref31/cit31 ref2/cit2 ref34/cit34 ref28/cit28 ref20/cit20 ref17/cit17 ref10/cit10 ref26/cit26 ref19/cit19 ref21/cit21 ref12/cit12 ref15/cit15 ref22/cit22 ref13/cit13 ref33/cit33 ref4/cit4 ref30/cit30 ref1/cit1 ref24/cit24 ref7/cit7 |
References_xml | – ident: ref7/cit7 doi: 10.1007/s11095-008-9697-x – ident: ref19/cit19 doi: 10.1007/s11095-004-9003-5 – ident: ref23/cit23 doi: 10.1007/s11095-011-0436-3 – ident: ref13/cit13 doi: 10.1073/pnas.0801763105 – ident: ref6/cit6 doi: 10.1146/annurev-bioeng-071811-150124 – ident: ref32/cit32 doi: 10.1103/PhysRevLett.99.138302 – ident: ref26/cit26 doi: 10.1016/S0168-3659(02)00180-3 – ident: ref33/cit33 doi: 10.1126/science.237.4813.384 – ident: ref34/cit34 doi: 10.1021/ma034506o – ident: ref2/cit2 doi: 10.1016/j.progpolymsci.2007.05.007 – ident: ref10/cit10 doi: 10.1002/anie.201104449 – ident: ref17/cit17 doi: 10.1002/adma.201202932 – ident: ref5/cit5 doi: 10.1016/j.addr.2009.04.016 – ident: ref1/cit1 doi: 10.1038/nrd1775 – ident: ref31/cit31 doi: 10.1103/PhysRevLett.99.138302 – ident: ref12/cit12 doi: 10.1038/nnano.2007.70 – ident: ref21/cit21 doi: 10.1021/bc025529v – ident: ref28/cit28 – ident: ref11/cit11 doi: 10.1021/nl052396o – ident: ref8/cit8 doi: 10.1073/pnas.1305000110 – ident: ref25/cit25 doi: 10.1016/0040-4039(95)00630-U – ident: ref27/cit27 doi: 10.1021/bm1009574 – ident: ref4/cit4 doi: 10.1098/rsif.2008.0547.focus – ident: ref24/cit24 doi: 10.1002/anie.200805895 – ident: ref16/cit16 doi: 10.1021/ma401093z – ident: ref30/cit30 doi: 10.1073/pnas.0705898104 – ident: ref20/cit20 doi: 10.1517/17425247.2013.744964 – ident: ref9/cit9 doi: 10.1073/pnas.1216893110 – ident: ref14/cit14 doi: 10.1073/pnas.1308345110 – ident: ref18/cit18 doi: 10.1002/jgm.868 – ident: ref3/cit3 doi: 10.1038/mt.2012.79 – ident: ref22/cit22 doi: 10.1146/annurev.pc.10.100159.000535 – ident: ref29/cit29 doi: 10.1063/1.470698 – ident: ref15/cit15 doi: 10.1021/nn403069n |
SSID | ssj0001385444 |
Score | 2.0609322 |
Snippet | Nanoparticles formed through complexation of plasmid DNA and copolymers are promising gene-delivery vectors, offering a wide range of advantages over... |
SourceID | pubmedcentral proquest crossref pubmed acs |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 448 |
Title | Simulation and Experimental Assembly of DNA–Graft Copolymer Micelles with Controlled Morphology |
URI | http://dx.doi.org/10.1021/acsbiomaterials.5b00080 https://www.ncbi.nlm.nih.gov/pubmed/29399627 https://search.proquest.com/docview/1994364350 https://pubmed.ncbi.nlm.nih.gov/PMC5796780 |
Volume | 1 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LT8JAEN4YvOjB9wNfWROPFunultKjQdCYwEEw8dbMtruRCMVAOejJ_-A_9Jc4uy0IGKOeu91mZ2Y737wJOROaAVOAtgkw6QgRaEd6XDmSK-BaSgDr0222Kjf34vbBe1gi7g8RfOZeQDQyleiQZhwpeVZvoZW-zEweoUFDtfaXW4VXPWFHuDLucwct6uokq-vnvYxmikbzmukb3FzMmpxRQ411cjcp5smyT55K41SWotfvvR3_fsINspaDUnqZSdEmWVLJFlmdaVW4TaDd7eeTvigkMa3PTAagJnLcl70XOtD0qnX58fZ-PQSd0pqZwPDSV0Pa7NoIwYgavy-tZfnxPRXT5gAZbV37O6TTqHdqN04-nsEBUXFTR6Kei2LlKUR8mmsEHmjaucJHCKg1SKEYQh1f6tgNIi8yag__F-BzVykWK813SSEZJGqfUO6LigTTqUYzodEmBxflJDBNS8uBDMpFco40CvPbNQpt4Jy54QLhwpxwRVKeMDJ8znp2_P7K6YThId4vQxJI1GCM3woCwRG2ebhmLxOA6aYIlQIzvKhI_DnRmC4wvbvnnyTdR9vD25QA-9Xywf9OdkhWELB5NlWtekQK6XCsjhEUpfLEXoNPPNgOpA |
link.rule.ids | 230,315,786,790,891,2782,27109,27957,27958,57093,57143 |
linkProvider | American Chemical Society |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lc9owEN5h6KHJIX0lLX1FnemxJtiSsH1kKJS0wAUyQ04eyZamTIPJYHOgp_6H_sP-kq5k8-x0MsnVlmVrd-X99qFdgI9Me8JTAm0T4UmHsVA7klPlSKoE1VIKYX26g2Gzd8W-TvikAsH6LAx-RIYzZTaIv60u4F7gNXMgXeQFY-rcqi801h9xH61yA4rao613hQac2U6uHvWpg4Z1sE7u-v9cRkHF2b6C-gd1HiZP7mij7hO43qzDJqH8qC9zWY9_HpR4fMhCn8JJCVFJq5CpZ1BR6XM43ilc-ALEaDor-34RkSaks9MngJg48kzerMhck8_D1p9fv78shM5J2_RjWM3UggymNl6QEeMFJu0iW_5GJWQwR7ZbR_8pjLudcbvnlM0aHMGabu5I1HpxorhC_KepRhiChp7LfASEWgvJlIfAx5c6ccOYx0YJ4t9D-NRVykuUpmdQTeepegWE-qwphalboz2m0UIXLkpNaEqYNkIZNmrwCWkUlXsti2wY3XOjA8JFJeFq0FjzM7otKnjc_ciHNd8j3G2GJCJV8yW-KwwZRRDHcczLQg42kyJwCk0roxr4exKyGWAqee_fSaffbUVvcyDYDxqv77eyc3jcGw_6Uf9y-O0NHCGU4zaJLXgL1XyxVO8QLuXyvd0ZfwGCFhcP |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1fb9owED8hKlXbQ7u220q3dZ7Ux4aR2CZE6ktFy1g70CSYxMsU2YmtoUJAJDy0T_0O-4b9JDs7gQLVNK2viePEd-fc7_74DuCEaU94SqBtIjzpMBZoR3KqHEmVoFpKIaxPt9Ott3-wqwEflOBscRYGPyLFmVIbxDe7ehrrosKA-xmvm0PpIsuZU-VWhaHBvsVNG28DjJq9Rw8LbXBmu7l61KcOGteNRYLX3-cySipK15XUE-S5mUC5opFau_BzuRabiHJTnWeyGt1tlHl87mJfwU4BVcl5Llt7UFLJPrxcKWB4AKI3HBf9v4hIYnK50i-AmHjyWI5uyUSTi-75w_3vLzOhM9I0fRlux2pGOkMbN0iJ8QaTZp41P1Ix6UyQ_dbh_xr6rct-s-0UTRscwepu5kjUflGsuEIcqKlGOIIGn8t8BIZaC8mUhwDIlzp2g4hHRhniX0T41FXKi5Wmb6CcTBJ1CIT6rC6FqV-jPabRUhcuSk9gSpnWAhnUKnCKNAqLPZeGNpzuueEG4cKCcBWoLXgaTvNKHv9-5NOC9yHuOkMSkajJHN8VBIwimOM45m0uC8tJEUAFpqVRBfw1KVkOMBW91-8kw1-2src5GOw3akf_t7KPsP39ohV--9q9fgcvENFxm8vWeA_lbDZXHxA1ZfLYbo4_KmEZiQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Simulation+and+Experimental+Assembly+of+DNA%E2%80%93Graft+Copolymer+Micelles+with+Controlled+Morphology&rft.jtitle=ACS+biomaterials+science+%26+engineering&rft.au=We%2C+Zonghui&rft.au=Ren%2C+Yong&rft.au=Williford%2C+John-Michael&rft.au=Qu%2C+Wei&rft.date=2015-06-08&rft.eissn=2373-9878&rft.volume=1&rft.issue=6&rft.spage=448&rft.epage=455&rft_id=info:doi/10.1021%2Facsbiomaterials.5b00080&rft_id=info%3Apmid%2F29399627&rft.externalDBID=PMC5796780 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2373-9878&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2373-9878&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2373-9878&client=summon |