An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo

A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic...

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Published in	Journal of the American Chemical Society Vol. 139; no. 26; pp. 8808 - 8811
Main Authors	Lozano-Torres, Beatriz, Galiana, Irene, Rovira, Miguel, Garrido, Eva, Chaib, Selim, Bernardos, Andrea, Muñoz-Espín, Daniel, Serrano, Manuel, Martínez-Máñez, Ramón, Sancenón, Félix
Format	Journal Article
Language	English
Published	WASHINGTON American Chemical Society 05.07.2017 Amer Chemical Soc
Subjects	Animals cell senescence Cellular Senescence - drug effects Chemistry Chemistry, Multidisciplinary drug therapy emissions factor fluorescence fluorescent dyes Fluorescent Dyes - chemistry galactose histidine Humans Mice Naphthalimides - chemistry Neoplasms - drug therapy nitrogen Photons Physical Sciences Reference Standards Science & Technology Xenograft Model Antitumor Assays VISUALIZATION TUMOR DESIGN BETA-GALACTOSIDASE ACTIVITY LIVING CELLS SCAFFOLD
Online Access	Get full text
ISSN	0002-7863 1520-5126 1520-5126
DOI	10.1021/jacs.7b04985

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Abstract	A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.
AbstractList	A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy. A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.
Author	Rovira, Miguel Serrano, Manuel Garrido, Eva Chaib, Selim Muñoz-Espín, Daniel Bernardos, Andrea Sancenón, Félix Lozano-Torres, Beatriz Galiana, Irene Martínez-Máñez, Ramón
AuthorAffiliation	Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM) Universitat Politècnica de València, IIS La Fe Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina Unidad Mixta de Investigación en Nanomedicina y Sensores Tumor Suppression Group CRUK Cancer Centre Early Detection Programme, Department of Oncology Universitat Politècnica de València, Centro de Investigación Príncipe Felipe
AuthorAffiliation_xml	– name: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM) – name: CRUK Cancer Centre Early Detection Programme, Department of Oncology – name: Universitat Politècnica de València, IIS La Fe – name: Universitat Politècnica de València, Centro de Investigación Príncipe Felipe – name: Unidad Mixta de Investigación en Nanomedicina y Sensores – name: Tumor Suppression Group – name: Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina
Author_xml	– sequence: 1 givenname: Beatriz surname: Lozano-Torres fullname: Lozano-Torres, Beatriz organization: Universitat Politècnica de València, IIS La Fe – sequence: 2 givenname: Irene surname: Galiana fullname: Galiana, Irene organization: Universitat Politècnica de València, IIS La Fe – sequence: 3 givenname: Miguel orcidid: 0000-0002-1391-8465 surname: Rovira fullname: Rovira, Miguel organization: Tumor Suppression Group – sequence: 4 givenname: Eva surname: Garrido fullname: Garrido, Eva organization: Universitat Politècnica de València, IIS La Fe – sequence: 5 givenname: Selim surname: Chaib fullname: Chaib, Selim organization: Tumor Suppression Group – sequence: 6 givenname: Andrea surname: Bernardos fullname: Bernardos, Andrea organization: Universitat Politècnica de València, IIS La Fe – sequence: 7 givenname: Daniel surname: Muñoz-Espín fullname: Muñoz-Espín, Daniel organization: CRUK Cancer Centre Early Detection Programme, Department of Oncology – sequence: 8 givenname: Manuel surname: Serrano fullname: Serrano, Manuel email: manuel.serrano@irbbarcelona.org organization: Tumor Suppression Group – sequence: 9 givenname: Ramón orcidid: 0000-0001-5873-9674 surname: Martínez-Máñez fullname: Martínez-Máñez, Ramón email: rmaez@qim.upv.es organization: Universitat Politècnica de València, IIS La Fe – sequence: 10 givenname: Félix surname: Sancenón fullname: Sancenón, Félix organization: Universitat Politècnica de València, IIS La Fe
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/28625064$$D View this record in MEDLINE/PubMed
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Snippet	A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl... A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an L-histidine methyl...
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SubjectTerms	Animals cell senescence Cellular Senescence - drug effects Chemistry Chemistry, Multidisciplinary drug therapy emissions factor fluorescence fluorescent dyes Fluorescent Dyes - chemistry galactose histidine Humans Mice Naphthalimides - chemistry Neoplasms - drug therapy nitrogen Photons Physical Sciences Reference Standards Science & Technology Xenograft Model Antitumor Assays
Title	An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo
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