An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo

• Published in: Journal of the American Chemical Society Vol. 139; no. 26; pp. 8808 - 8811
• Main Authors: Lozano-Torres, Beatriz, Galiana, Irene, Rovira, Miguel, Garrido, Eva, Chaib, Selim, Bernardos, Andrea, Muñoz-Espín, Daniel, Serrano, Manuel, Martínez-Máñez, Ramón, Sancenón, Félix
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 05.07.2017; Amer Chemical Soc
• Subjects: Animals; cell senescence; Cellular Senescence - drug effects; Chemistry; Chemistry, Multidisciplinary; drug therapy; emissions factor; fluorescence; fluorescent dyes; Fluorescent Dyes - chemistry; galactose; histidine; Humans; Mice; Naphthalimides - chemistry; Neoplasms - drug therapy; nitrogen; Photons; Physical Sciences; Reference Standards; Science & Technology; Xenograft Model Antitumor Assays; VISUALIZATION; TUMOR; DESIGN; BETA-GALACTOSIDASE ACTIVITY; LIVING CELLS; SCAFFOLD
• ISSN: 0002-7863; 1520-5126; 1520-5126
• DOI: 10.1021/jacs.7b04985

A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.