Fast Photochemical Oxidation of Proteins for Comparing Structures of Protein−Ligand Complexes: The Calmodulin−Peptide Model System

• Published in: Analytical chemistry (Washington) Vol. 83; no. 1; pp. 311 - 318
• Main Authors: Zhang, Hao, Gau, Brian C, Jones, Lisa M, Vidavsky, Ilan, Gross, Michael L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.01.2011
• Subjects: Amino Acid Sequence; Amino acids; Analytical chemistry; Animals; Calcium - chemistry; Calcium - metabolism; Calmodulin - chemistry; Calmodulin - metabolism; Cattle; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid; Comparative analysis; Exact sciences and technology; Hydrogen peroxide; Kinetics; Ligands; Models, Molecular; Molecular Sequence Data; Musculoskeletal system; Other chromatographic methods; Oxidation; Oxidation-Reduction; Peptides; Peptides - chemistry; Peptides - metabolism; Photochemical Processes; Protein Conformation; Proteins; Spectrometric and optical methods; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Hydrogen peroxide; Peptides; Ligand; Biochemistry; Characterization; Photolysis; Proteolysis; Oxidation; Solvent; Calmodulin; Quantitative analysis; Drug; Use; Liquid chromatography; Exposure; Method; Striated muscle; Microsecond; Protein; Residue; Aminoacid; Mass spectrometry MS/MS; Laser; Mass spectrometry; Comparative study

Fast photochemical oxidation of proteins (FPOP) is a mass spectrometry-based protein footprinting method that modifies proteins on the microsecond time scale. Highly reactive •OH, produced by laser photolysis of hydrogen peroxide, oxidatively modifies the side chains of approximately one-half the co...