Variation in the metagenomic analysis of fecal microbiome composition calls for a standardized operating approach

• Published in: Microbiology spectrum Vol. 12; no. 12; p. e0151624
• Main Authors: Xu, Zhilu, Yeoh, Yun Kit, Tun, Hein M., Fei, Na, Zhang, Jingwan, Morrison, Mark, Kamm, Michael A., Yu, Jun, Chan, Francis Ka Leung, Ng, Siew C.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 30.10.2024
• Subjects: batch effect; DNA extraction; Human Microbiome; metagenomics; microbiome; Research Article; batch effect; DNA extraction; microbiome; metagenomics

The reproducibility of human gut microbiome studies has been suboptimal across cohorts and study design choices. One possible reason for the disagreement is the introduction of systemic biases due to differences in methodologies. In our study, we utilized microbial metagenomic data sets from 2,722 f...