Quantification and Identification of Microproteinuria Using Ultrafiltration and ATR-FTIR Spectroscopy

• Published in: Analytical chemistry (Washington) Vol. 92; no. 3; pp. 2409 - 2416
• Main Authors: Perez-Guaita, David, Richardson, Zack, Heraud, Philip, Wood, Bayden
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.02.2020
• Subjects: absorbance; Albumin; Albumins; calibration; Chemistry; data collection; detection limit; Diagnosis; films (materials); Fourier analysis; Fourier transform infrared spectroscopy; Fourier transforms; Healthy Volunteers; Hemoglobin; Humans; Identification methods; Illnesses; image analysis; immunoglobulins; Infrared spectroscopy; Linearity; Multivariate analysis; neoplasms; point-of-care systems; prediction; Prognosis; Proteins; Proteinuria - urine; Renal failure; Spectroscopy, Fourier Transform Infrared; Thin films; Ultracentrifugation; Ultrafiltration; Urine

The presence of low amounts of specific proteins in urine can be an indicator of diagnosis and prognosis of several diseases including renal failure and cancer. Hence, there is an urgent need for Point-of-care (PoC) methods, which can quantify microproteinuria levels (30–300 ppm) and identify the ma...