In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients
Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how in vivo bacterial functioning differs from i...
Saved in:
Published in | Journal of proteome research Vol. 18; no. 6; pp. 2601 - 2612 |
---|---|
Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
07.06.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how in vivo bacterial functioning differs from in vitro conditions, we investigated the in vivo proteomes of PA in 35 sputum samples from 11 CF patients. We developed a novel bacterial-enrichment method that relies on differential centrifugation and detergent treatment to enrich for bacteria to improve identification of PA proteome with CF sputum samples. Using two nonredundant peptides as a cutoff, a total of 1304 PA proteins were identified directly from CF sputum samples. The in vivo PA proteomes were compared with the proteomes of ex vivo-grown PA populations from the same patient sample. Label-free quantitation and proteome comparison revealed the in vivo up-regulation of siderophore TonB-dependent receptors, remodeling in central carbon metabolism including glyoxylate cycle and lactate utilization, and alginate overproduction. Knowledge of these in vivo proteome differences or others derived using the presented methodology could lead to future treatment strategies aimed at altering PA physiology in vivo to compromise infectivity or improve antibiotic efficacy. |
---|---|
AbstractList | Chronic airway infection with
P. aeruginosa
(PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how
in vivo
bacterial functioning differs from
in vitro
conditions, we investigated the
in vivo
proteomes of PA in 35 sputum samples from 11 CF patients. We developed a novel bacterial-enrichment method that relies on differential centrifugation and detergent treatment to enrich for bacteria to improve identification of PA proteome with CF sputum samples. Using two non-redundant peptides as a cutoff, a total of 1304 PA proteins were identified directly from CF sputum samples. The
in vivo
PA proteomes were compared with the proteomes of
ex vivo
-grown PA populations from the same patient sample. Label-free quantitation and proteome comparison revealed the
in vivo
up-regulation of siderophore TonB-dependent receptors, remodeling in central carbon metabolism including glyoxylate cycle and lactate utilization, and alginate overproduction. Knowledge of these
in vivo
proteome differences or others derived using the presented methodology could lead to future treatment strategies aimed at altering PA physiology
in vivo
to compromise infectivity or improve antibiotic efficacy. Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how in vivo bacterial functioning differs from in vitro conditions, we investigated the in vivo proteomes of PA in 35 sputum samples from 11 CF patients. We developed a novel bacterial-enrichment method that relies on differential centrifugation and detergent treatment to enrich for bacteria to improve identification of PA proteome with CF sputum samples. Using two nonredundant peptides as a cutoff, a total of 1304 PA proteins were identified directly from CF sputum samples. The in vivo PA proteomes were compared with the proteomes of ex vivo-grown PA populations from the same patient sample. Label-free quantitation and proteome comparison revealed the in vivo up-regulation of siderophore TonB-dependent receptors, remodeling in central carbon metabolism including glyoxylate cycle and lactate utilization, and alginate overproduction. Knowledge of these in vivo proteome differences or others derived using the presented methodology could lead to future treatment strategies aimed at altering PA physiology in vivo to compromise infectivity or improve antibiotic efficacy. |
Author | Staudinger, Benjamin J Bruce, James E Ozer, Egon A Hisert, Katherine B Singh, Pradeep K Siehnel, Richard J Hauser, Alan R Eng, Jimmy K Garudathri, Jayanthi Manoil, Colin Wu, Xia |
AuthorAffiliation | Department of Chemistry Department of Medicine Department of Genome Sciences Department of Microbiology-Immunology Department of Microbiology |
AuthorAffiliation_xml | – name: Department of Microbiology – name: – name: Department of Chemistry – name: Department of Genome Sciences – name: Department of Medicine – name: Department of Microbiology-Immunology – name: 3 Department of Medicine, University of Washington, Seattle, WA, USA – name: 1 Department of Genome Sciences, University of Washington, Seattle, WA, USA – name: 6 Department of Medicine, Northwestern University, Chicago, Illinois, USA – name: 4 Department of Chemistry, University of Washington, Seattle, WA, USA – name: 2 Department of Microbiology, University of Washington, Seattle, WA, USA – name: 5 Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA |
Author_xml | – sequence: 1 givenname: Xia surname: Wu fullname: Wu, Xia – sequence: 2 givenname: Richard J surname: Siehnel fullname: Siehnel, Richard J – sequence: 3 givenname: Jayanthi surname: Garudathri fullname: Garudathri, Jayanthi – sequence: 4 givenname: Benjamin J surname: Staudinger fullname: Staudinger, Benjamin J – sequence: 5 givenname: Katherine B surname: Hisert fullname: Hisert, Katherine B – sequence: 6 givenname: Egon A surname: Ozer fullname: Ozer, Egon A – sequence: 7 givenname: Alan R surname: Hauser fullname: Hauser, Alan R – sequence: 8 givenname: Jimmy K surname: Eng fullname: Eng, Jimmy K – sequence: 9 givenname: Colin surname: Manoil fullname: Manoil, Colin – sequence: 10 givenname: Pradeep K surname: Singh fullname: Singh, Pradeep K – sequence: 11 givenname: James E orcidid: 0000-0001-6441-6089 surname: Bruce fullname: Bruce, James E email: jimbruce@u.washington.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31060355$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkU9PKyEUxYnR-P8jaFi6ab3A3JmyMTGN-kxMXk3ULWEYqpgOVJip6beXvtZGV28FCb9z7uWcI7Lrg7eEnDEYMuDsUps0fJ_H0NnQ2qGsARjnO-SQocCBkFDtft9HUhyQo5TeM4IViH1yIBiUIBAPyeO9py9uEehkY0XDlE6S7ZvQBq8T1Tb2r86HpKnz9NrFT71MK2i8TJ0z9NbVMSSX6ER3zvounZC9qZ4le7o5j8nz7c3T-M_g4e_d_fj6YaALFN2gkoaXo0KIBm0ppTGc2worNKibBtAWhahRjrTkpmg4QgNMspLB1NRCywLEMbla-877urWNybOjnql5dK2OSxW0U79fvHtTr2GhygoBALPBxcYgho_epk61Lhk7m2lvQ58U54KvokaeUVyjJv81RTvdjmGgVozKdahtHWpTR9ad_9xxq_rOPwNsDfzThz76HNl_TL8AazKdqQ |
CitedBy_id | crossref_primary_10_1080_14789450_2023_2258282 crossref_primary_10_1099_mgen_0_000513 crossref_primary_10_1038_s41579_020_00477_5 crossref_primary_10_1016_j_bios_2019_111538 crossref_primary_10_3389_fcimb_2024_1346813 crossref_primary_10_3390_metabo9100234 crossref_primary_10_3390_antibiotics10111393 crossref_primary_10_1128_JB_00307_19 crossref_primary_10_3390_ph13090214 crossref_primary_10_1016_j_jmb_2020_07_021 crossref_primary_10_1021_jasms_9b00144 crossref_primary_10_3390_cells8101129 crossref_primary_10_3389_fcimb_2021_724569 crossref_primary_10_1371_journal_pbio_3002694 crossref_primary_10_3389_fmicb_2022_1006946 crossref_primary_10_1038_s41522_019_0113_6 crossref_primary_10_1073_pnas_2107644118 crossref_primary_10_3389_fmicb_2021_640787 crossref_primary_10_1016_j_ijbiomac_2023_128563 crossref_primary_10_3390_ijms23105692 crossref_primary_10_3390_vaccines11010028 |
Cites_doi | 10.1126/science.1211037 10.1099/mic.0.030858-0 10.1016/j.jprot.2016.10.005 10.1128/JB.01307-07 10.1038/nprot.2015.053 10.1021/pr200019m 10.1002/3527603875.ch4 10.1164/rccm.201312-2142OC 10.1183/09031936.03.00043603 10.1038/ng.3148 10.1016/j.jprot.2013.04.009 10.1128/iai.62.2.596-605.1994 10.1128/IAI.00144-10 10.1038/cdd.2017.101 10.1128/JB.00259-16 10.1183/09031936.01.17100270 10.1128/IAI.01530-06 10.1093/bioinformatics/btq054 10.1073/pnas.95.25.14863 10.1073/pnas.0602138103 10.1073/pnas.1422186112 10.1016/j.chom.2015.07.006 10.1038/ncomms13414 10.1016/j.femsim.2004.11.010 10.1164/ajrccm/141.4_Pt_1.914 10.1038/35037627 10.1021/pr200659h 10.1016/j.chembiol.2015.09.015 10.1073/pnas.1717525115 10.1128/JB.01581-07 10.1128/JB.00166-09 10.1164/rccm.201609-1954OC 10.1371/journal.pone.0062917 10.1002/pmic.200900375 10.1111/j.1365-2958.1992.tb01407.x 10.1046/j.1365-2958.2000.01846.x 10.1111/1462-2920.12155 10.1186/1471-2164-15-737 10.1128/IAI.73.11.7151-7160.2005 10.1126/science.1155207 10.1016/j.ijmm.2013.07.009 10.1073/pnas.0407460101 10.1038/srep13139 10.1128/mr.60.3.539-574.1996 10.1099/13500872-145-6-1349 10.1136/thorax.55.9.795 10.1086/318075 10.1073/pnas.1104984108 10.1007/s10529-006-9156-x 10.1128/JB.01138-07 10.1073/pnas.1018249108 10.1038/nbt1001-946 10.1128/AEM.01015-12 10.1093/nar/gkv1145 10.3389/fcimb.2013.00075 10.1038/ncomms8996 10.1104/pp.106.078006 10.1126/science.1097524 10.1046/j.1365-313X.1995.07010165.x 10.1186/s12859-018-2154-x 10.1038/s41467-018-05944-5 10.1074/mcp.M113.033985 10.1128/JB.185.3.1071-1081.2003 10.1136/thx.2006.060889 10.1128/JB.188.1.45-54.2006 10.1093/nar/gks1094 10.1099/13500872-140-7-1745 10.1128/IAI.01807-06 10.1128/JB.187.15.5267-5277.2005 10.1002/pmic.201200439 10.1002/ppul.21198 10.1093/nar/gkq869 10.1099/mic.0.2007/014506-0 10.1016/S1044-0305(03)00133-8 10.1128/CMR.00036-10 10.1128/MMBR.00027-10 10.1186/s40248-015-0031-2 10.1126/scitranslmed.aat7520 10.1002/pmic.201100515 10.1038/ncomms7001 10.1016/j.ymben.2016.09.002 10.1371/journal.pone.0147698 10.1128/JB.183.18.5395-5401.2001 10.1074/mcp.M115.050161 10.1093/nar/gku469 10.1128/jb.143.2.872-878.1980 10.1371/journal.pbio.1001242 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1021/acs.jproteome.9b00122 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 1535-3907 |
EndPage | 2612 |
ExternalDocumentID | 10_1021_acs_jproteome_9b00122 31060355 a739515133 |
Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NIAID NIH HHS grantid: U19 AI107775 – fundername: NCRR NIH HHS grantid: S10 RR025107 – fundername: NIDDK NIH HHS grantid: P30 DK089507 – fundername: NIAID NIH HHS grantid: R01 AI101307 – fundername: NHLBI NIH HHS grantid: R01 HL110879 – fundername: NIGMS NIH HHS grantid: R01 GM086688 |
GroupedDBID | - 53G 55A 5GY 7~N AABXI ABMVS ABUCX ACGFS ACS AEESW AENEX AFEFF ALMA_UNASSIGNED_HOLDINGS AQSVZ CS3 DU5 EBS ED ED~ EJD F5P GNL IH9 IHE JG JG~ P2P RNS ROL UI2 VF5 VG9 W1F ZA5 --- 4.4 5VS AAHBH ABJNI ABQRX ADHLV AHGAQ BAANH CGR CUPRZ CUY CVF ECM EIF GGK NPM AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-a453t-79c268433d5e699cc22e7575c5add05e443b598a92c4d250d0191610fcb3a9403 |
IEDL.DBID | ACS |
ISSN | 1535-3893 |
IngestDate | Tue Sep 17 21:12:09 EDT 2024 Fri Oct 25 01:45:58 EDT 2024 Fri Dec 06 01:56:25 EST 2024 Sat Sep 28 08:27:09 EDT 2024 Thu Aug 27 13:43:31 EDT 2020 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Keywords | Pseudomonas aeruginosa cystic fibrosis quantitative proteomics bacterial population proteomes |
Language | English |
License | https://doi.org/10.15223/policy-029 https://doi.org/10.15223/policy-037 https://doi.org/10.15223/policy-045 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-a453t-79c268433d5e699cc22e7575c5add05e443b598a92c4d250d0191610fcb3a9403 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0001-6441-6089 |
OpenAccessLink | https://europepmc.org/articles/pmc6750005?pdf=render |
PMID | 31060355 |
PQID | 2232102152 |
PQPubID | 23479 |
PageCount | 12 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_6750005 proquest_miscellaneous_2232102152 crossref_primary_10_1021_acs_jproteome_9b00122 pubmed_primary_31060355 acs_journals_10_1021_acs_jproteome_9b00122 |
ProviderPackageCode | JG~ 55A AABXI GNL VF5 7~N VG9 W1F ACS AEESW AFEFF ABMVS ABUCX IH9 AQSVZ ED~ UI2 |
PublicationCentury | 2000 |
PublicationDate | 2019-06-07 |
PublicationDateYYYYMMDD | 2019-06-07 |
PublicationDate_xml | – month: 06 year: 2019 text: 2019-06-07 day: 07 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Journal of proteome research |
PublicationTitleAlternate | J. Proteome Res |
PublicationYear | 2019 |
Publisher | American Chemical Society |
Publisher_xml | – name: American Chemical Society |
References | ref9/cit9 ref45/cit45 ref3/cit3 ref27/cit27 ref81/cit81 ref63/cit63 ref56/cit56 Govan J. R. (ref73/cit73) 1996; 60 ref23/cit23 Hancock R. E. W. (ref52/cit52) 2005 ref8/cit8 ref31/cit31 ref59/cit59 ref85/cit85 ref2/cit2 ref77/cit77 ref34/cit34 ref71/cit71 ref37/cit37 ref20/cit20 ref48/cit48 ref60/cit60 ref74/cit74 ref17/cit17 Nicas T. I. (ref64/cit64) 1980; 143 ref82/cit82 ref10/cit10 ref35/cit35 ref53/cit53 ref19/cit19 ref21/cit21 ref42/cit42 ref46/cit46 ref49/cit49 ref13/cit13 ref61/cit61 ref75/cit75 ref67/cit67 ref24/cit24 ref38/cit38 ref50/cit50 ref78/cit78 ref54/cit54 ref6/cit6 ref36/cit36 ref18/cit18 ref83/cit83 ref65/cit65 ref79/cit79 ref11/cit11 ref25/cit25 ref29/cit29 ref72/cit72 ref76/cit76 ref86/cit86 ref32/cit32 ref39/cit39 ref14/cit14 ref57/cit57 ref5/cit5 ref51/cit51 ref43/cit43 ref80/cit80 ref28/cit28 ref40/cit40 ref68/cit68 ref26/cit26 ref55/cit55 ref69/cit69 ref12/cit12 ref15/cit15 ref62/cit62 ref66/cit66 Mahenthiralingam E. (ref16/cit16) 1994; 62 ref41/cit41 ref58/cit58 ref22/cit22 ref33/cit33 ref87/cit87 ref4/cit4 ref30/cit30 ref47/cit47 ref84/cit84 ref1/cit1 ref44/cit44 ref70/cit70 ref7/cit7 |
References_xml | – ident: ref13/cit13 doi: 10.1126/science.1211037 – ident: ref69/cit69 doi: 10.1099/mic.0.030858-0 – ident: ref25/cit25 doi: 10.1016/j.jprot.2016.10.005 – ident: ref78/cit78 doi: 10.1128/JB.01307-07 – ident: ref81/cit81 doi: 10.1038/nprot.2015.053 – ident: ref30/cit30 doi: 10.1021/pr200019m – start-page: 61 volume-title: Bacterial and Eukaryotic Porins year: 2005 ident: ref52/cit52 doi: 10.1002/3527603875.ch4 contributor: fullname: Hancock R. E. W. – ident: ref6/cit6 doi: 10.1164/rccm.201312-2142OC – ident: ref44/cit44 doi: 10.1183/09031936.03.00043603 – ident: ref19/cit19 doi: 10.1038/ng.3148 – ident: ref32/cit32 doi: 10.1016/j.jprot.2013.04.009 – volume: 62 start-page: 596 issue: 2 year: 1994 ident: ref16/cit16 publication-title: Infect. Immun. doi: 10.1128/iai.62.2.596-605.1994 contributor: fullname: Mahenthiralingam E. – ident: ref46/cit46 doi: 10.1128/IAI.00144-10 – ident: ref40/cit40 doi: 10.1038/cdd.2017.101 – ident: ref76/cit76 doi: 10.1128/JB.00259-16 – ident: ref45/cit45 doi: 10.1183/09031936.01.17100270 – ident: ref71/cit71 doi: 10.1128/IAI.01530-06 – ident: ref41/cit41 doi: 10.1093/bioinformatics/btq054 – ident: ref86/cit86 doi: 10.1073/pnas.95.25.14863 – ident: ref3/cit3 doi: 10.1073/pnas.0602138103 – ident: ref82/cit82 doi: 10.1073/pnas.1422186112 – ident: ref18/cit18 doi: 10.1016/j.chom.2015.07.006 – ident: ref31/cit31 doi: 10.1038/ncomms13414 – ident: ref54/cit54 doi: 10.1016/j.femsim.2004.11.010 – ident: ref84/cit84 doi: 10.1164/ajrccm/141.4_Pt_1.914 – ident: ref5/cit5 doi: 10.1038/35037627 – ident: ref22/cit22 doi: 10.1021/pr200659h – ident: ref47/cit47 doi: 10.1016/j.chembiol.2015.09.015 – ident: ref12/cit12 doi: 10.1073/pnas.1717525115 – ident: ref8/cit8 doi: 10.1128/JB.01581-07 – ident: ref72/cit72 doi: 10.1128/JB.00166-09 – ident: ref28/cit28 doi: 10.1164/rccm.201609-1954OC – ident: ref85/cit85 doi: 10.1371/journal.pone.0062917 – ident: ref38/cit38 doi: 10.1002/pmic.200900375 – ident: ref58/cit58 doi: 10.1111/j.1365-2958.1992.tb01407.x – ident: ref77/cit77 doi: 10.1046/j.1365-2958.2000.01846.x – ident: ref7/cit7 doi: 10.1111/1462-2920.12155 – ident: ref34/cit34 doi: 10.1186/1471-2164-15-737 – ident: ref17/cit17 doi: 10.1128/IAI.73.11.7151-7160.2005 – ident: ref70/cit70 doi: 10.1126/science.1155207 – ident: ref57/cit57 doi: 10.1016/j.ijmm.2013.07.009 – ident: ref21/cit21 doi: 10.1073/pnas.0407460101 – ident: ref66/cit66 doi: 10.1038/srep13139 – volume: 60 start-page: 539 issue: 3 year: 1996 ident: ref73/cit73 publication-title: Microbiol Rev. doi: 10.1128/mr.60.3.539-574.1996 contributor: fullname: Govan J. R. – ident: ref50/cit50 doi: 10.1099/13500872-145-6-1349 – ident: ref51/cit51 doi: 10.1136/thorax.55.9.795 – ident: ref14/cit14 doi: 10.1086/318075 – ident: ref63/cit63 doi: 10.1073/pnas.1104984108 – ident: ref75/cit75 doi: 10.1007/s10529-006-9156-x – ident: ref83/cit83 doi: 10.1128/JB.01138-07 – ident: ref20/cit20 doi: 10.1073/pnas.1018249108 – ident: ref39/cit39 doi: 10.1038/nbt1001-946 – ident: ref65/cit65 doi: 10.1128/AEM.01015-12 – ident: ref42/cit42 doi: 10.1093/nar/gkv1145 – ident: ref53/cit53 doi: 10.3389/fcimb.2013.00075 – ident: ref59/cit59 doi: 10.1038/ncomms8996 – ident: ref49/cit49 doi: 10.1104/pp.106.078006 – ident: ref62/cit62 doi: 10.1126/science.1097524 – ident: ref48/cit48 doi: 10.1046/j.1365-313X.1995.07010165.x – ident: ref37/cit37 doi: 10.1186/s12859-018-2154-x – ident: ref11/cit11 doi: 10.1038/s41467-018-05944-5 – ident: ref27/cit27 doi: 10.1074/mcp.M113.033985 – ident: ref79/cit79 doi: 10.1128/JB.185.3.1071-1081.2003 – ident: ref29/cit29 doi: 10.1136/thx.2006.060889 – ident: ref60/cit60 doi: 10.1128/JB.188.1.45-54.2006 – ident: ref87/cit87 doi: 10.1093/nar/gks1094 – ident: ref74/cit74 doi: 10.1099/13500872-140-7-1745 – ident: ref10/cit10 doi: 10.1128/IAI.01807-06 – ident: ref9/cit9 doi: 10.1128/JB.187.15.5267-5277.2005 – ident: ref33/cit33 doi: 10.1002/pmic.201200439 – ident: ref2/cit2 doi: 10.1002/ppul.21198 – ident: ref36/cit36 doi: 10.1093/nar/gkq869 – ident: ref68/cit68 doi: 10.1099/mic.0.2007/014506-0 – ident: ref23/cit23 doi: 10.1016/S1044-0305(03)00133-8 – ident: ref1/cit1 doi: 10.1128/CMR.00036-10 – ident: ref80/cit80 doi: 10.1128/MMBR.00027-10 – ident: ref55/cit55 doi: 10.1186/s40248-015-0031-2 – ident: ref56/cit56 doi: 10.1126/scitranslmed.aat7520 – ident: ref43/cit43 doi: 10.1002/pmic.201100515 – ident: ref67/cit67 doi: 10.1038/ncomms7001 – ident: ref4/cit4 doi: 10.1016/j.ymben.2016.09.002 – ident: ref26/cit26 doi: 10.1371/journal.pone.0147698 – ident: ref15/cit15 doi: 10.1128/JB.183.18.5395-5401.2001 – ident: ref24/cit24 doi: 10.1074/mcp.M115.050161 – ident: ref35/cit35 doi: 10.1093/nar/gku469 – volume: 143 start-page: 872 issue: 2 year: 1980 ident: ref64/cit64 publication-title: J. Bacteriol. doi: 10.1128/jb.143.2.872-878.1980 contributor: fullname: Nicas T. I. – ident: ref61/cit61 doi: 10.1371/journal.pbio.1001242 |
SSID | ssj0015703 |
Score | 2.4415262 |
Snippet | Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain... Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain... |
SourceID | pubmedcentral proquest crossref pubmed acs |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 2601 |
SubjectTerms | Adult Anti-Bacterial Agents - therapeutic use Bacterial Proteins - genetics Carbon - metabolism Cystic Fibrosis - diagnosis Cystic Fibrosis - drug therapy Cystic Fibrosis - genetics Cystic Fibrosis - microbiology Female Glyoxylates - metabolism Humans Lactic Acid - metabolism Male Membrane Proteins - genetics Middle Aged Proteome - genetics Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa - pathogenicity Pseudomonas Infections - diagnosis Pseudomonas Infections - drug therapy Pseudomonas Infections - genetics Pseudomonas Infections - microbiology Sputum - microbiology |
Title | In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients |
URI | http://dx.doi.org/10.1021/acs.jproteome.9b00122 https://www.ncbi.nlm.nih.gov/pubmed/31060355 https://search.proquest.com/docview/2232102152 https://pubmed.ncbi.nlm.nih.gov/PMC6750005 |
Volume | 18 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1bS8MwFD54edAX75d5I4JPQmvXNs3yKMMxBWXiBd9KmmZYxVaWVZm_3pNehlOG-tomoTnnpOf7OMkXgCO3Hzd5TGOLI5-wfOYHlmiJpsUEdwv8HRQHhS-vgu6df_FAH2bgZEoF322eCKntp0K0IHtRNi-LQbMw7zKk4AYLtW_GZQMjJ1UKpFLLZOL6yM60YUxKknoyJf3Amd-3S37JP51l6NWneMptJ892Poxs-fFT1PGvU1uBpQqLktMyeFZhRqVrsNCur4Bbh-vzlNwnbxnpVb1J1ic9rfI4w-gVmgg1yPEDMi1IkpLTZPAuRto0ao-M_jPpIBnPdKJJr5Rv1Rtw1zm7bXet6g4GS_jUG1qMS6MH43kxVQHnUrquYgjxJMUfo0OV73sR5S30rPRjhFMxQkYEkU5fRp7gvuNtwlyapWobCBJxGjv9gCGFQdjmRNgr4i1HMS9whGQNOEaDhNUa0mFRHnebYfGwtlJYWakBdu2z8LXU5fitw2Ht2RCtaMoiIlVZrkMESIb3IpBpwFbp6fGQCH4DByFZA9hEDIwbGHXuyTdp8liodCMTM4B45z-z2oVFXCG82IrG9mBuOMjVPoKeYXRQBPonUDX-kQ |
link.rule.ids | 230,314,780,784,885,2765,27076,27924,27925,56738,56788 |
linkProvider | American Chemical Society |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3dT9swED8BeygvjPHZDYYn7QkpJU3iuH5EFVXZaNVp5eMtchxXBESC6mYT--s5O0lZQQjx6tiWfXfO_U53_hnguzdJ2jyhicMxnnACFoSO6Ii2wwT3LP4O7UXhwTDsnwc_rujVEoT1XRhchMaZtE3iP7ELtI9M243lLsjvVIuXOaFl-EDNs5UGEnV_z7MHhlWq5EmljnHI9c2d16YxnknqRc_0Am4-r5r8zw31PsLlfAO2-uS2Vczilvz3jNvx_Ttch7UKmZLj0pQ-wZLKNqDRrR-E24Rfpxm5SP_kZFSNJvmEjLQqkhxtWWgi1LTAdeRakDQjx-n0r3jQplP3wbBBkx6G5rlONRmVZK56C857J-Nu36leZHBEQP2Zw7g07DC-n1AVci6l5ymGgE9S_E26VAWBH1PeQT3LIEFwlSCAREjpTmTsCx64_jasZHmmdoFgWE4TdxIyDGgQxLkxjop5x1XMD10hWRMOUSBRdaJ0ZJPlXjuyjbWUokpKTWjVqovuS5aOtwZ8qxUcoRRNkkRkKi90hHDJRMEIa5qwUyp8PiVC4dBFgNYEtmAK8w6Gq3vxS5ZeW85ujMsMPP78nl0dQKM_HpxFZ6fDn19gFc8Ot0VqbA9WZtNC7SMcmsVfre0_AhVfBw0 |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Rb9MwED5BkWAvMBhsBQaexNOkdGkSx_VjVahaYFOnsWkSD5FjOyKbllR1Axq_nrOTVHTTNMGrE1v23dn3ne78GeBDkKk-V1R5HOMJL2JR7ImB6HtM8MDh79hdFD48iien0edzet5UVdq7MDgJgyMZl8S3u3qusoZhoH9g2y8cf0F5pXu8zgs9hEcUT1xbzjUcnawyCJZZquZKpZ51yu3tnbuGsd5JmnXvdAty3qyc_MsVjZ_B99UiXAXKZa9apj35-wa_4_-tchOeNgiVDGuTeg4PdPECnozah-G24HhakLP8Z0lmTW9SZmRmdKVKtGlhiNCLCudSGkHyggzzxS9xbexPo2vLCk3GGKKXJjdkVpO6mpdwOv70bTTxmpcZPBHRcOkxLi1LTBgqqmPOpQwCzRD4SYrHpU91FIUp5QPUt4wUgiyFQBKhpZ_JNBQ88sNX0CnKQu8AwfCcKj-LGQY2COb8FHulfOBrFsa-kKwL-yiQpNlZJnFJ86CfuMZWSkkjpS70WvUl85qt474Oe62SE5SiTZaIQpeVSRA22WgY4U0Xtmulr4ZESBz7CNS6wNbMYfWD5exe_1LkPxx3N8ZnFia__pdVvYfHs4_j5Ov06Msb2MAtxF2tGnsLneWi0ruIipbpO2f-fwCAxQmQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+Vivo+Proteome+of+Pseudomonas+aeruginosa+in+Airways+of+Cystic+Fibrosis+Patients&rft.jtitle=Journal+of+proteome+research&rft.au=Wu%2C+Xia&rft.au=Siehnel%2C+Richard+J&rft.au=Garudathri%2C+Jayanthi&rft.au=Staudinger%2C+Benjamin+J&rft.date=2019-06-07&rft.eissn=1535-3907&rft.volume=18&rft.issue=6&rft.spage=2601&rft.epage=2612&rft_id=info:doi/10.1021%2Facs.jproteome.9b00122&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1535-3893&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1535-3893&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1535-3893&client=summon |