SAPT, a Fast and Efficient Approach for Simultaneous Profiling of Protein N- and C‑Terminome

• Published in: Analytical chemistry (Washington) Vol. 93; no. 30; pp. 10553 - 10560
• Main Authors: Wang, Zhongjie, Zhang, Lei, Yuan, Wenjuan, Zhang, Ying, Lu, Haojie
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 03.08.2021
• Subjects: Acetylation; analytical chemistry; Biological activity; Cation exchange; cation exchange chromatography; Cation exchanging; Chemistry; data collection; Datasets; Fractionation; human cell lines; humans; Peptides; Protein C; Proteins; Selectivity; Workflow

Protein termini play pivotal roles in various biological processes. Although several terminomic strategies have been proposed for the analysis of protein N-termini or protein C-termini separately, few can analyze both ends of proteins at the same time. Herein, we developed a workflow, termed Simulta...