Protein Structural Denaturation Evaluated by MCR-ALS of Protein Microarray FTIR Spectra

• Published in: Analytical chemistry (Washington) Vol. 93; no. 40; pp. 13441 - 13449
• Main Authors: De Meutter, Joëlle, Goormaghtigh, Erik
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 12.10.2021
• Subjects: Agglomeration; analytical chemistry; Chemistry; Denaturation; Feature recognition; Fourier transform infrared spectroscopy; Fourier transforms; Infrared analysis; Infrared imaging; Infrared spectroscopy; Protein arrays; Protein denaturation; Protein interaction; protein microarrays; Protein structure; Proteins; Secondary structure; Spectra

The loss of native structure is common in proteins. Among others, aggregation is one structural modification of particular importance as it is a major concern for the efficiency and safety of biotherapeutic proteins. Yet, recognizing the structural features associated with intermolecular bridging in...