SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV‑2 in Clinical Samples

The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role i...

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Published inACS applied bio materials Vol. 4; no. 4; pp. 2974 - 2995
Main Authors Yadav, Shalu, Sadique, Mohd. Abubakar, Ranjan, Pushpesh, Kumar, Neeraj, Singhal, Ayushi, Srivastava, Avanish K, Khan, Raju
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 19.04.2021
Subjects
Antibodies, Immobilized - chemistry
Antibodies, Immobilized - immunology
Antibodies, Viral - blood
Antibodies, Viral - immunology
Biomarkers - blood
Biomarkers - metabolism
COVID-19 - diagnosis
COVID-19 - virology
Humans
Immunoassay - methods
Point-of-Care Systems
Review
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - metabolism
Spectrum Analysis, Raman
Viral Proteins - immunology
Viral Proteins - metabolism
COVID-19
POC devices
SARS-CoV-2
lateral flow immunoassay
surface-enhanced Raman spectroscopy
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Abstract The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
AbstractList The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
Author Kumar, Neeraj
Yadav, Shalu
Srivastava, Avanish K
Khan, Raju
Sadique, Mohd. Abubakar
Ranjan, Pushpesh
Singhal, Ayushi
AuthorAffiliation Academy of Scientific and Innovative Research (AcSIR)
Microfluidics & MEMS Centre
AuthorAffiliation_xml – name: Microfluidics & MEMS Centre
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  organization: Academy of Scientific and Innovative Research (AcSIR)
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  surname: Kumar
  fullname: Kumar, Neeraj
  organization: Academy of Scientific and Innovative Research (AcSIR)
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  email: khan.raju@gmail.com, khan.raju@ampri.res.in
  organization: Academy of Scientific and Innovative Research (AcSIR)
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35014387$$D View this record in MEDLINE/PubMed
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Issue 4
Keywords COVID-19
POC devices
SARS-CoV-2
lateral flow immunoassay
surface-enhanced Raman spectroscopy
Language English
License https://doi.org/10.15223/policy-017
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Snippet The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid,...
The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid,...
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SubjectTerms Antibodies, Immobilized - chemistry
Antibodies, Immobilized - immunology
Antibodies, Viral - blood
Antibodies, Viral - immunology
Biomarkers - blood
Biomarkers - metabolism
COVID-19 - diagnosis
COVID-19 - virology
Humans
Immunoassay - methods
Point-of-Care Systems
Review
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - metabolism
Spectrum Analysis, Raman
Viral Proteins - immunology
Viral Proteins - metabolism
Title SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV‑2 in Clinical Samples
URI http://dx.doi.org/10.1021/acsabm.1c00102
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