Loading of Antibiotic into Biocoated Hydroxyapatite Nanoparticles: Smart Antitumor Platforms with Regulated Release

In this research we propose a nanoplatform for anticancer therapy that is based on the combination of three components: (1) an antibiotic to target selectively the mitochondria of cancer cells, inhibiting their functions; (2) mineral nanoparticles (NPs) able to encapsulate the antibiotic and to ente...

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Published inACS biomaterials science & engineering Vol. 4; no. 9; pp. 3234 - 3245
Main Authors Rivas, Manuel, del Valle, Luís J, Rodríguez-Rivero, Anna M, Turon, Pau, Puiggalí, Jordi, Alemán, Carlos
Format Journal Article Publication
LanguageEnglish
Published United States American Chemical Society 10.09.2018
American Chemical Society (ACS)
Subjects
antibiotic
Antibiotics
Antibiòtics
biocoating
Calcium phosphate
Cancer
Cancer cells
Chloramphenicol
Cloramfenicol
Càncer
Cèl·lules canceroses
Enginyeria química
Fosfat de calci
polyphosphate
Àrees temàtiques de la UPC
antibiotic
polyphosphate
biocoating
cancer cells
chloramphenicol
calcium phosphate
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Abstract In this research we propose a nanoplatform for anticancer therapy that is based on the combination of three components: (1) an antibiotic to target selectively the mitochondria of cancer cells, inhibiting their functions; (2) mineral nanoparticles (NPs) able to encapsulate the antibiotic and to enter into the cells across the cell membrane; and (3) a biocoating to protect the antibiotic during and/or after its regulated release, increasing its therapeutic efficacy. Chloramphenicol (CAM), a prototypical wide-spectrum antibiotic, has been used to induce mitochondrial-dysfunctions in cancer cells. Different in situ synthetic strategies have been tested to load such antibiotic into both crystalline hydroxyapatite (cHAp) and amorphous calcium phosphate (ACP) NPs. cHAp NPs showed higher loading capacity, in terms of encapsulation and superficial adsorption of CAM, and slower antibiotic release than ACP NPs. On the other hand, the protecting role played by biocoatings based on pyrophosphate and, especially, triphosphate was greater than that of biophosphonates, the anticancer therapeutic efficacy of CAM being maximized by the former. In vitro studies using healthy and cancer cell lines have demonstrated that in situ CAM-loaded cHAp NPs coated with triphosphate selectively kill a great population of cancer cells, evidencing the potential of this nanoplatform in cancer treatment.
AbstractList In this research we propose a nanoplatform for anticancer therapy that is based on the combination of three components: (1) an antibiotic to target selectively the mitochondria of cancer cells, inhibiting their functions; (2) mineral nanoparticles (NPs) able to encapsulate the antibiotic and to enter into the cells across the cell membrane; and (3) a biocoating to protect the antibiotic during and/or after its regulated release, increasing its therapeutic efficacy. Chloramphenicol (CAM), a prototypical wide-spectrum antibiotic, has been used to induce mitochondrial-dysfunctions in cancer cells. Different synthetic strategies have been tested to load such antibiotic into both crystalline hydroxyapatite (cHAp) and amorphous calcium phosphate (ACP) NPs. cHAp NPs showed higher loading capacity, in terms of encapsulation and superficial adsorption of CAM, and slower antibiotic release than ACP NPs. On the other hand, the protecting role played by biocoatings based on pyrophosphate and, especially, triphosphate was greater than that of biophosphonates, the anticancer therapeutic efficacy of CAM being maximized by the former. studies using healthy and cancer cell lines have demonstrated that CAM-loaded cHAp NPs coated with triphosphate selectively kill a great population of cancer cells, evidencing the potential of this nanoplatform in cancer treatment.
In this research we propose a nanoplatform for anticancer therapy that is based on the combination of three components: (1) an antibiotic to target selectively the mitochondria of cancer cells, inhibiting their functions; (2) mineral nanoparticles (NPs) able to encapsulate the antibiotic and to enter into the cells across the cell membrane; and (3) a biocoating to protect the antibiotic during and/or after its regulated release, increasing its therapeutic efficacy. Chloramphenicol (CAM), a prototypical wide-spectrum antibiotic, has been used to induce mitochondrial-dysfunctions in cancer cells. Different in situ synthetic strategies have been tested to load such antibiotic into both crystalline hydroxyapatite (cHAp) and amorphous calcium phosphate (ACP) NPs. cHAp NPs showed higher loading capacity, in terms of encapsulation and superficial adsorption of CAM, and slower antibiotic release than ACP NPs. On the other hand, the protecting role played by biocoatings based on pyrophosphate and, especially, triphosphate was greater than that of biophosphonates, the anticancer therapeutic efficacy of CAM being maximized by the former. In vitro studies using healthy and cancer cell lines have demonstrated that in situ CAM-loaded cHAp NPs coated with triphosphate selectively kill a great population of cancer cells, evidencing the potential of this nanoplatform in cancer treatment.
In this research we propose a nanoplatform for anticancer therapy that is based on the combination of three components: (1) an antibiotic to target selectively the mitochondria of cancer cells, inhibiting their functions; (2) mineral nanoparticles (NPs) able to encapsulate the antibiotic and to enter into the cells across the cell membrane; and (3) a biocoating to protect the antibiotic during and/or after its regulated release, increasing its therapeutic efficacy. Chloramphenicol (CAM), a prototypical wide-spectrum antibiotic, has been used to induce mitochondrial-dysfunctions in cancer cells. Different in situ synthetic strategies have been tested to load such antibiotic into both crystalline hydroxyapatite (cHAp) and amorphous calcium phosphate (ACP) NPs. cHAp NPs showed higher loading capacity, in terms of encapsulation and superficial adsorption of CAM, and slower antibiotic release than ACP NPs. On the other hand, the protecting role played by biocoatings based on pyrophosphate and, especially, triphosphate was greater than that of biophosphonates, the anticancer therapeutic efficacy of CAM being maximized by the former. In vitro studies using healthy and cancer cell lines have demonstrated that in situ CAM-loaded cHAp NPs coated with triphosphate selectively kill a great population of cancer cells, evidencing the potential of this nanoplatform in cancer treatment. Peer Reviewed
Author Puiggalí, Jordi
Turon, Pau
Rivas, Manuel
del Valle, Luís J
Rodríguez-Rivero, Anna M
Alemán, Carlos
AuthorAffiliation B. Braun Surgical
Barcelona Research Center for Multiscale Science and Engineering
Departament d’Enginyeria Química, EEBE
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  organization: Barcelona Research Center for Multiscale Science and Engineering
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33435064$$D View this record in MEDLINE/PubMed
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cancer cells
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calcium phosphate
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SubjectTerms antibiotic
Antibiotics
Antibiòtics
biocoating
Calcium phosphate
Cancer
Cancer cells
Chloramphenicol
Cloramfenicol
Càncer
Cèl·lules canceroses
Enginyeria química
Fosfat de calci
polyphosphate
Àrees temàtiques de la UPC
Title Loading of Antibiotic into Biocoated Hydroxyapatite Nanoparticles: Smart Antitumor Platforms with Regulated Release
URI http://dx.doi.org/10.1021/acsbiomaterials.8b00353
https://www.ncbi.nlm.nih.gov/pubmed/33435064
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