Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase
When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinit...
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| Published in | Biochemistry (Easton) Vol. 28; no. 7; pp. 2768 - 2771 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Chemical Society
04.04.1989
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| Subjects | |
| Online Access | Get full text |
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| Abstract | When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum. |
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| AbstractList | When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum. When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of {sup 3}H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of he label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at 1:1 ratio with molybdenum. |
| Author | Imperial, Juan Shah, Vinod K Hoover, Timothy R Ludden, Paul W |
| Author_xml | – sequence: 1 givenname: Timothy R surname: Hoover fullname: Hoover, Timothy R – sequence: 2 givenname: Juan surname: Imperial fullname: Imperial, Juan – sequence: 3 givenname: Paul W surname: Ludden fullname: Ludden, Paul W – sequence: 4 givenname: Vinod K surname: Shah fullname: Shah, Vinod K |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2663059$$D View this record in MEDLINE/PubMed https://www.osti.gov/biblio/5673222$$D View this record in Osti.gov |
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| Snippet | When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into... When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of {sup 3}H-labeled homocitrate, label was incorporated... |
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| SubjectTerms | 550201 - Biochemistry- Tracer Techniques BASIC BIOLOGICAL SCIENCES BIOSYNTHESIS CARBOXYLIC ACID SALTS Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Ion Exchange CITRATES COENZYMES ENZYMES Ferredoxins - biosynthesis HYDROGEN COMPOUNDS Kinetics Klebsiella pneumoniae - enzymology MAGNETIC RESONANCE Magnetic Resonance Spectroscopy Molybdoferredoxin - biosynthesis Molybdoferredoxin - isolation & purification NITRO-GROUP DEHYDROGENASES NITROGENASE Nitrogenase - biosynthesis Nitrogenase - isolation & purification NUCLEAR MAGNETIC RESONANCE OXIDOREDUCTASES PURIFICATION RESONANCE SYNTHESIS Tricarboxylic Acids - analysis Tricarboxylic Acids - metabolism TRITIUM COMPOUNDS UPTAKE |
| Title | Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase |
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