Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons

• Published in: Journal of agricultural and food chemistry Vol. 58; no. 20; pp. 10864 - 10868
• Main Authors: Sánchez-Campillo, María, Pérez-Llamas, Francisca, González-Silvera, Daniel, Martínez-Tomás, Rebeca, Burgos, M. Isabel, Wellner, Anna, Avilés, Francisco, Parra, Soledad, Bialek, Lucy, Alminger, Marie, Larqué, Elvira
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 27.10.2010
• Subjects: Adult; antioxidant activity; Antioxidants - analysis; Antioxidants - metabolism; beta-carotene; Bioactive Constituents; bioavailability; Biochemistry - methods; blood chemistry; carotenoids; Carotenoids - analysis; Carotenoids - metabolism; chylomicron; chylomicrons; Chylomicrons - analysis; Chylomicrons - isolation & purification; Chylomicrons - metabolism; dietary; Female; foods; Fruit - metabolism; Hep G2 Cells; HepG2 cells; human cell lines; Humans; lycopene; metabolism; oxidative stress; plasma; postprandial; Postprandial Period; quantitative analysis; Reactive oxygen species; remnants; salmonella-typhimurium; tomato lycopene; vegetable and fruit food; vegetables; Vegetables - metabolism; Young Adult; postprandial; Reactive oxygen species; lycopene; chylomicron; β-carotene; vegetable and fruit food; HepG2 cells

We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup...