Direct relationship between protein expression and progeny yield of herpes simplex virus 1

Earlier single-cell studies of virus-infected cells have revealed high heterogeneity in the state of viral gene expression and progeny virus yield. Notably, these two aspects have been shown independently, and therefore, the direct relationship between progeny virus production and viral gene express...

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Published inmBio Vol. 16; no. 6; p. e0028025
Main Authors Nobe, Moeka, Maruzuru, Yuhei, Takeshima, Kosuke, Maeda, Fumio, Kusano, Hideo, Yoshimura, Raiki, Nishiyama, Takara, Park, Hyeongki, Kozaki, Yoshitaka, Iwami, Shingo, Koyanagi, Naoto, Kato, Akihisa, Natsume, Tohru, Adachi, Shungo, Kawaguchi, Yasushi
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 11.06.2025
Subjects
Animals
Cell Line
Chlorocebus aethiops
Gene Expression Regulation, Viral
Genes, Reporter
herpes simplex virus
Herpesvirus 1, Human - genetics
Herpesvirus 1, Human - physiology
heterogeneity of infection
Humans
Nucleocapsid - metabolism
rate-limiting step for virus production
Research Article
Vero Cells
Viral Proteins - biosynthesis
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
Virus Replication
herpes simplex virus
rate-limiting step for virus production
heterogeneity of infection
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Summary:Earlier single-cell studies of virus-infected cells have revealed high heterogeneity in the state of viral gene expression and progeny virus yield. Notably, these two aspects have been shown independently, and therefore, the direct relationship between progeny virus production and viral gene expression has been unclear. This study, for the first time, demonstrated the direct and quantitative relationship between viral protein expression and progeny virus production by taking into account their heterogeneities and revealed a threshold for the levels of herpes simplex virus 1 protein expression for progeny virus production, thereby suggesting the existence of a rate-limiting step in progeny virus production.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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The authors declare no conflict of interest.
Moeka Nobe and Yuhei Maruzuru contributed equally to this article. The authors are listed in order of increasing seniority.
ISSN:2150-7511
2150-7511
DOI:10.1128/mbio.00280-25