Tertiary Interactions between the Fifth and Sixth Transmembrane Segments of Rhodopsin

• Published in: Biochemistry (Easton) Vol. 38; no. 20; pp. 6597 - 6603
• Main Authors: Struthers, Mary, Yu, Hongbo, Kono, Masahiro, Oprian, Daniel D.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 18.05.1999
• Subjects: Absorption; Amino Acid Substitution - genetics; Animals; Cattle; Cell Membrane - chemistry; Cell Membrane - metabolism; Cross-Linking Reagents - chemistry; Cysteine - genetics; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Mutagenesis, Site-Directed; Peptide Fragments - chemistry; Peptide Fragments - metabolism; Phenylalanine - genetics; Protein Structure, Tertiary; Rhodopsin - chemistry; Rhodopsin - metabolism; Spectrophotometry; Transducin - metabolism

We have used cysteine scanning mutagenesis and disulfide cross-linking in a split rhodopsin construct to investigate the secondary structure and tertiary contacts of the fifth (TM5) and sixth (TM6) transmembrane segments of rhodopsin. Using a simple increase in pH to promote disulfide bond formation...